MAB4301

Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480

clone MC-480, Chemicon^®, from mouse

• Synonyme(s) : SSEA-1, CD15, Lewis X, 3-FAL
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: saturated ammonium sulfate (SAS) precipitated
• Type de produit anticorps: primary antibodies
• Clone: MC-480, monoclonal
• Espèces réactives: mouse, human, rat
• Conditionnement: antibody small pack of 25 μg
• Fabricant/nom de marque: Chemicon^®
• Technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• Entrée: sample type: mouse embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type neural stem cell(s); sample type: human embryonic stem cell(s)
• Isotype: IgM
• Numéro d'accès NCBI: NM_002033.2
• Numéro d'accès UniProt: P22083
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FUT4(2526)

Description

Description générale

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Spécificité

This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Immunogène

F9 tetracarcinoma stem cells (X-irradiated).

Application

Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480 detects level of Stage-Specific Embryonic Antigen-1 & has been published & validated for use in FC, IF, IH & IP.

Immunohistochemistry:
A previous lot of this antibody was tested on IH.

Immunoprecipitation:
A previous lot of this antibody was tested on IP.

Immunofluorescence:
A previous lot of this antibody was tested on IF. 4% PFA fixed, 5-10 minutes room temperature. No detergent is required as epitope is external.

Flow Cytometry:
A starting range of 10-20 µg/mL is suggested.

Positive mouse EC lines include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Lines : PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147 were negative.

Optimal working dilutions must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Neural Stem Cells

Qualité

Tested

Description de la cible

220 kDa

Forme physique

Format: Purified

Purified mouse monoclonal IgM in buffer containing 0.05M Potassium Phosphate, 0.3M NaCl, pH 8.0 with 0.05% Sodium Azide

Stockage et stabilité

Stable for 1 year at 2-8ºC from date of receipt.

Remarque sur l'analyse

Control
P19 mouse embryonic carcinoma cells

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable