ABS994

Anti-phospho-Glucocorticoid receptor Antibody, Ser226

from rabbit, purified by affinity chromatography

• Synonyme(s) : Nuclear receptor subfamily 3 group C member 1, glucocorticoid receptor, GR
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_000167
• Numéro d'accès UniProt: P04150
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer226)
• Informations sur le gène: human ... NR3C1(2908)

Description

Description générale

Glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1, and encoded by the gene name NR3C1/ GRL, is receptor for glucocorticoids (GC). GR has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. GR affects inflammatory responses, cellular proliferation and differentiation in target tissues. Recent studies have shown that GR may act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. In addition, GR is involved in chromatin remodeling, and may play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression. Mutation in GR has been associated with Glucocorticoid resistance (GCRES): a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. GR is widely expressed. In the heart, GR is detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart.

Immunogène

Epitope: A region within the modulating domain

KLH-conjugated linear peptide corresponding to the domain of: Modulating region of Human Glucocorticoid receptor.

Application

Research Category
Signaling

Research Sub Category
Lipid Metabolism & Weight Regulation

This Anti-phospho-Glucocorticoid receptor antibody is validated for use in Western Blotting for the detection of phospho-Glucocorticoid receptor.

Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in transfected U2OS cell lysate (Chen, W., et al. Mol Endocrinol, August 2008, 22(8):1754–1766)
Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in rat hepatoma cell lysate (Blind,R., et al. Journal of Steroid Biochemistry & Molecular Biology 109 (2008) 150–157)

Qualité

Evaluated by Western Blotting on transfected HEK293 cell lysate

Western Blotting Analysis: 1 µg/mL dilution of this antibody detected phosphorylated glucocorticoid receptor in 6 µg of transfected HEK293 cell lysate

Description de la cible

~85 kDa observed

Forme physique

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable