344906

FURA 2/AM in Solution

• Synonyme(s) : FURA 2/AM in Solution
• Formule empirique (notation de Hill): C₄₄H₄₇N₃O₂₄
• Numéro CAS: 108964-32-5
• Poids moléculaire : 1001.85
• Numéro MDL: MFCD00036976
• Code UNSPSC : 12171500
• Nomenclature NACRES : NA.47

Propriétés

• Pureté: ≥95% (HPLC)
• Niveau de qualité: 100
• Forme: liquid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Fluorescence: λ_ex 335 nm (high); λ_ex 362 nm (low); λ_em 505 nm (high); λ_em 512 nm (low)
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3
• Clé InChI: UTBYGIDPBQOHNT-UHFFFAOYSA-N

Description

Description générale

Cell-permeable ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (K_d = 224 nM) that offers 30X greater fluorescence intensity that QUIN 2 (Cat. No. 551826). This probe has been used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm).

Actions biochimiques/physiologiques

Cell permeable: no

Product does not compete with ATP.

Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Irritant (B)

Forme physique

A 1 mM solution of FURA 2/AM (Cat. No. 344905) in anhydrous DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Store frozen under an inert atmosphere.

Autres remarques

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Point d'éclair (°C): 87 °C - closed cup - (Dimethylsulfoxide)