This product has not been specifically tested for freeze/thaw tolerance in solution. However, product 540025, which is Pluronic F-127 10% solution in water, is suitable for freezing. A 10% solution of Pluronic F-127 in phosphate-buffered saline is expected to be stable when stored in aliquots at –20°C. Avoid repeated freeze/thaw cycles.
P2443
Pluronic® F-127
powder, BioReagent, suitable for cell culture
Synonyme(s) :
Non-ionic copolymer surfactant
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A propos de cet article
Formule linéaire :
(C3H6O·C2H4O)x
Numéro CAS:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NA.75
MDL number:
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description
Non-ionic, contains 100ppm BHT
product line
BioReagent
form
powder
mol wt
~12600 g/mol
technique(s)
cell culture | mammalian: suitable
CMC
950-1000 ppm (~25°C)
transition temp
cloud point >100 °C
HLB
18 - 23
SMILES string
O2C(C2)C.O1CC1
InChI
1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2
InChI key
RVGRUAULSDPKGF-UHFFFAOYSA-N
General description
Pluronic® F-127, également appelé poloxamère 407, est un copolymère non ionique et non toxique[1]. Il contient de l'oxyde de polyéthylène et de l'oxyde de propylène disposés en une structure tribloc formant une chaîne PEO-PPO-PEO symétrique. Les propriétés tensioactives de ce polymère, qui découlent de sa nature amphiphile, lui permettent d'interagir avec les surfaces hydrophobes et les membranes biologiques.
Application
Pluronic® F-127 a été utilisé :
- comme système d'administration pour un peptide sur le site d'une plaie afin d'étudier l'effet du peptide sur la cicatrisation chez des souris.
- comme enduction de surface sur des timbres en PDMS (polydiméthylsiloxane) pour leur conférer des propriétés répulsives vis-à-vis d'hydrogels[2].
- dans la préparation d'une solution-mère indicatrice de calcium afin de mesurer des activités neurales.
- comme constituant d'une solution saline tamponnée au phosphate (PBS), pour réduire l'adhérence non spécifique des cellules et des protéines à un dispositif microfluidique à base de PDMS[3].
- Ce tensioactif non ionique de type copolymère peut être utilisé comme agent antimoussant dans la culture de cellules d'insectes.
Biochem/physiol Actions
Pluronic® F-127 est un gel thermoréversible utilisé dans différentes industries en raison de sa grande thermosensibilité, de sa biodégradabilité élevée et de son importante biocompatibilité.[1] Ces propriétés font de ce polymère un véhicule polyvalent pour les médicaments. Pluronic® F-127 améliore l'efficacité thérapeutique des médicaments en augmentant leur biodisponibilité et leur thermostabilité. Ce produit peut en outre promouvoir l'adhésion cellulaire et la formation de collagène, ce qui facilite l'angiogenèse et la régénération tissulaire. En raison de son efficacité, Pluronic F-127 a été utilisé pour encapsuler des cellules souches mésenchymateuses (CSM) afin de faciliter la régénération de tissus faiblement vascularisés tels que les tissus épithéliaux, les tissus adipeux, le cartilage, les tendons et les tissus osseux.[1]
Legal Information
Pluronic is a registered trademark of BASF
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Cet article | |||
|---|---|---|---|
| form powder | form - | form solid | form solid, waxy solid |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) cell culture | mammalian: suitable |
| mol wt ~12600 g/mol | mol wt - | mol wt - | mol wt - |
| product line BioReagent | product line - | product line - | product line - |
| HLB 18 - 23 | HLB - | HLB - | HLB - |
| Quality Level 200 | Quality Level 100 | Quality Level - | Quality Level 100 |
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Contenu apparenté
Philippe Marmottant et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(41), 17271-17275 (2009-10-07)
Cell aggregates are a tool for in vitro studies of morphogenesis, cancer invasion, and tissue engineering. They respond to mechanical forces as a complex rather than simple liquid. To change an aggregate's shape, cells have to overcome energy barriers. If
Connexin43 carboxyl-terminal peptides reduce scar progenitor and promote regenerative healing following skin wounding
Ghatnekar GS, et al.
Regenerative Medicine, 4(2), 205-223 (2009)
Federico Ceriani et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(46), E7194-E7201 (2016-11-04)
Spatially and temporally coordinated variations of the cytosolic free calcium concentration ([Ca2+]c) play a crucial role in a variety of tissues. In the developing sensory epithelium of the mammalian cochlea, elevation of extracellular adenosine trisphosphate concentration ([ATP]e) triggers [Ca2+]c oscillations
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| P2443-250G | 04061834363200 |
| P2443-1KG | 04061835569076 |
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Can I aliquot and freeze pluronic 10% (in PBS) at -20°C?
1 answer-
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Pluroni F-127 어떤 solution에 녹여서 stock 만들어야 되나요? D.W 인가요? DMSO 인가요? PBS 인가요?
1 answer-
This surfactant is soluble in water at >10% at room temperature. The product solubility is tested at 50 mg/mL in water. Similar rates are expected in PBS. The solubility in DMSO has not been investigated.
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Is Pluronic® F-127 soluble in water and/or KOH solutions (up to 10M)?
1 answer-
This product is tested for solubility at 50 mg/mL in water at temperatures less than 10°C. Maximum solubility has not been tested.
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What is the hydroxyl number of F127?
1 answer-
Based on an approximate molecular weight of 12,600, this block co-polymer with two hydroxyl groups has a theoretical hydroxyl value of 8.9 mg potassium hydroxide (KOH) per gram of polymer. Higher experimental results for the hydroxyl value would indicate some branching.
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Is there a reference for using Pluronic F-127 to reduce cell adhesion in a microfluidic PDMS-based device? Also, does it dissolve in cell culture media in a PDMS-based device, and if it does, what modifications are recommended to maintain its ability to prevent cell adhesion?
1 answer-
There are several representative publications that report the use of Pluronic F-127 to lower cell adhesion on microfluidic PDMS-based devices. These publications may be of interest as a reference:
Curr Protoc Cell Biol. 2017 Jun 19;75:10.21.1-10.21.8. Microcontact Peeling: A Cell Micropatterning Technique for Circumventing Direct Adsorption of Proteins to Hydrophobic PDMS. PMID: 28627756
Sci Rep. 2019 May 14;9(1):7377. Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics. PMID: 31089162
Lab Chip. 2009 Jun 7;9(11):1500-3. Surface modification of PDMS by gradient-induced migration of embedded Pluronic. PMID: 19458853
These publications provide information on using Pluronic F-127 to lower cell adhesion on PDMS-based devices. As for its solubility in cell culture media within a PDMS-based device and the recommended modifications to maintain its ability to prevent cell adhesion, it is advised to refer to these publications for detailed insights.
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I was asked about the polydispersity of Pluronic F-127, Product P2443. Could you please inform me?
1 answer-
The polydispersity is not a test specification for this product. The product is an ABA-type copolymer of poly (ethylene oxide) (PEO=A) and poly (propylene oxide) (PPO=B), where the A and B blocks have the following values: A = 101, B = 56. The approximate relative amount of PEO in the poloxamer is ca. 70% and the average molecular weight of the PPO is 4000. The molecular structure is shown below:
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can I know what is the chain length of each PEO and PPO of this F-127?
1 answer-
Pluronic F 127 corresponds to the structure of Kolliphor P 407.
This product is an ABA-type copolymer of polyethylene oxide (PEO=A) and polypropylene oxide (PPO=B), where the A and B blocks have the following values:
A = 101
B = 56Helpful?
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What is the VOC content of this product?
1 answer-
Volatile Organic Compound (VOC) content is not measured for this product.
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Une fois le Pluronic® F-127 mis en solution (à 10% dans de l'eau par exemple), combien de temps et à quelle température se conserve-t-il ?
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The solubility of the product is 50 mg/ml water, at less than 10°C. The stability of the product once diluted has not been tested, in which case it is suggested to prepare the necessary quantity before use.
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What is the best way to sterilise Pluronic F127 dissolved in water so that it is suitable for cell culture?
1 answer-
The solution can be sterilized with a 0.22 uM sterile filter.
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