176872

Angiotensin Converting Enzyme-2, His•Tag^®, Human, Recombinant, NSO Cells

• Synonyme(s) : ACE Homolog, ACEH, ACE-2
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in NSO cells
• Niveau de qualité: 100
• Pureté: ≥90% (SDS-PAGE)
• Forme: liquid
• Activité spécifique: ≥0.8 units/μg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Impuretés: ≤1.0 EU/μg Endotoxins (EU/μg ACE-2)
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. ACE-2 cleaves angiotensins I and II as a carboxypeptidase and plays an important role in regulating cardiovascular and renal functions. Native ACE-2 is an integral membrane zinc metalloprotease whose activity is inhibited by EDTA. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing conditions.

Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. Native ACE-2 is an integral membrane zinc metalloprotease that can be inhibited by EDTA. ACE-2 cleaves angiotensins I and II. Plays an important role in regulating cardiovascular and renal functions. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing condition.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-YVADAPK(DNP)-OH per min at 25°C, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Tikellis, C., et al. 2003. Hypertension41, 390.
Tipnis, S.R., et al. 2000. J. Biol. Chem.275, 33238.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable