1.08218

Trifluoroacetic acid solution

(25% solution in water) for protein sequenation

• Synonyme(s) : Trifluoroacetic acid solution, TFA solution, TFA
• Formule linéaire : CF₃COOH
• Numéro CAS: 76-05-1
• Poids moléculaire : 114.02
• Numéro Beilstein : 5446934
• Numéro MDL: MFCD00004169
• Code UNSPSC : 12352106
• Nomenclature NACRES : NB.21

Propriétés

• Niveau de qualité: 200
• Forme: liquid
• Composition: Water, 74.5-75.5% Karl Fischer
• Concentration: 24.5-25.5% (trifluoroacetic acid)
• Densité: 1.12 g/cm³ at 20 °C
• Température de stockage: 15-25°C
• Chaîne SMILES: OC(=O)C(F)(F)F
• InChI: 1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
• Clé InChI: DTQVDTLACAAQTR-UHFFFAOYSA-N

Description

Application

Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Remarque sur l'analyse

Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H314 - H412
• Conseils de prudence: P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A
• Code de la classe de stockage: 8B - Non-combustible, corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable