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Merck
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Key Documents

07-453

Sigma-Aldrich

Anti-B-Raf Antibody

Upstate®, from rabbit

Synonyme(s) :

Anti-B-RAF1, Anti-B-raf, Anti-BRAF-1, Anti-BRAF1, Anti-NS7, Anti-RAFB1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

human, rat, mouse

Fabricant/nom de marque

Upstate®

Technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

NM_004333

Numéro d'accès UniProt

P15056

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... BRAF(673)
mouse ... Braf(109880)
rat ... Rasd1(64455)

Spécificité

full length recombinant BRaf (plus 6His tag) and B-Raf splice variants in cell lysates

Immunogène

synthetic peptide corresponding to amino acids 754-765 of human B-Raf

Application

Research Category
Signaling
Research Sub Category
MAP Kinases
This Anti-B-Raf Antibody is validated for use in IP, WB for the detection of B-Raf. This B-Raf antibody has been tested in Human, Mouse, and Rat.

Qualité

routinely evaluated by immunoblot in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme

Description de la cible

94kDa

Liaison

Replaces: 04-328

Forme physique

0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein A chromatography

Stockage et stabilité

2 years at -20°C

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Gang Ren et al.
International journal of cell biology, 2012, 913242-913242 (2012-05-23)
Recent high-throughput-sequencing of the cancer genome has identified oncogenic mutations in BRaf genetic locus as one of the critical events in melanomagenesis. In normal cells, the activity of BRaf is tightly regulated. Gain-of-function mutations like those identified in melanoma frequently
Lack of association between BRAF V600E mutation and mitogen-activated protein kinase activation in papillary thyroid carcinoma.
Hui Zuo,Yasushi Nakamura,Hironao Yasuoka,Ping Zhang,Misa Nakamura,Ichiro Mori et al.
Pathology International null
Sandeep S Joshi et al.
PloS one, 13(2), e0191264-e0191264 (2018-02-27)
Heat shock protein 90 (HSP90) is a molecular chaperone which stabilizes client proteins with important roles in tumor growth. 17-allylamino-17-demethoxygeldanamycin (17-AAG), an inhibitor of HSP90 ATPase activity, occupies the ATP binding site of HSP90 causing a conformational change which destabilizes
Regulation of B-Raf kinase activity by tuberin and Rheb is mammalian target of rapamycin (mTOR)-independent.
Karbowniczek, M; Cash, T; Cheung, M; Robertson, GP; Astrinidis, A; Henske, EP
The Journal of Biological Chemistry null
Anne K Voss et al.
The EMBO journal, 25(15), 3652-3663 (2006-07-22)
The mechanisms regulating the size of the cerebral cortex are poorly understood. Here, we demonstrate that the Rap1 guanine nucleotide exchange factor, C3G (Grf2, Rapgef1), controls the size of the cerebral precursor population. Mice lacking C3G show overproliferation of the
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