05-1570

Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3

clone 6D3, from rat

• Synonyme(s) : PR domain containing 1, with ZNF domain, PR-domain zinc finger protein 1, beta-interferon gene positive-regulatory domain I binding factor, Positive regulatory domain I-binding factor 1, PRDI-binding factor-1, Beta-interferon gene positive regulatory dom
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 6D3, monoclonal
• Espèces réactives: mouse, human
• Technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_031574
• Numéro d'accès UniProt: Q60636
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PRDM1(639)

Description

Description générale

B-lymphocyte induced maturation protein (BLIMP-1) is a 100 kDa transcriptional repressor containing 1 DET domain and 4 C2H2-type zinc fingers. In the nucleus, BLIMP-1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, BLIMP-1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade.

Spécificité

Recognizes BLIMP-1

Immunogène

Epitope: Recognizes epitope located between amino acids 255-395 of BLIMP-1

Recombinant protein corresponding to mouse BLIMP-1.

Application

ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)

Flow Cytometry Analysis: A representative lot was used by an independent laboratory in FC. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)

Research Category
Epigenetics & Nuclear Function

Inflammation & Immunology

Use Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 (Rat Monoclonal Antibody) validated in WB, ELISA, IHC, FC to detect PRDM1 (BLIMP-1) also known as PR-domain zinc finger protein 1.

Qualité

Evaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected BLIMP-1 on 10 µg of HL-60 cell lysate.

Description de la cible

~ 95 kDa observed

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable