C8104
Monoclonal Anti-Ceramide antibody produced in mouse
clone MID 15B4, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Ceramide Antibody, Ceramide Antibody - Monoclonal Anti-Ceramide antibody produced in mouse
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
MID 15B4, monoclonal
Forme
buffered aqueous solution
Technique(s)
immunohistochemistry (frozen sections): suitable
indirect ELISA: 1:10
Isotype
IgM
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Description générale
Ceramide is an endogenous lipid component of a novel biochemical pathway termed the sphingomyelin pathway. Ceramide is produced in response to cellular stimulation by hormones, cytokines and antigens. Ceramide synthesis is mediated either by the salvage pathway by the acylation of sphingosine or sphingolipids hydrolysis or by de novo pathway via dihydroceramide formation. Structurally, ceramides have a sphingoid base, a long-chain amino alcohol linked to fatty acid by an amide bond. Ceramide is generated by hydrolysis of sphingomyelin by sphingomyelinase.
Spécificité
Mouse monoclonal clone MID 15B4 anti-Ceramide antibody recognizes free and bound ceramides.
Immunogène
ceramide conjugated to bovine serum albumin.
Application
Monoclonal Anti-Ceramide antibody produced in mouse has been used in:
- immunohistochemistry
- immunolabeling in electron microscopy
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- as a probe to determine the presence and roles of ceramide in sphingomyelin pathway signaling and the regulation of protein phosphorylation.
Actions biochimiques/physiologiques
Ceramide appears to have a role in mediating biological responses in a wide variety of cell types. Ceramide metabolites such as sphingosine and sphingosine-1-phosphate have potent biological activities of their own. They are directly involved in the proliferation and differentiation of skin cells and regulate skin barrier functionality. Ceramide based analogs are potential anti-tumor agents and are regarded as tumor suppressor lipid. Mechanisms for ceramide action involve regulation of protein phosphorylation via stimulation of a serine/threonine protein phosphatase, a proline-directed kinase and possibly other direct and/or indirect targets. Ceramide is emerging as an intracellular messenger than mediates effects on terminal differentiation and cell proliferation as well as apoptosis or cell death and cell-cycle arrest. The interrelationship of ceramide actions with other bioactive lipids and systems represents an area of active research.
Forme physique
Solution in phosphate buffered saline containing 0.5 M NaCl, 0.1% bovine serum albumin, and 0.09% sodium azide.
Notes préparatoires
Purified from ascites fluid by gel filtration using sephacryl S-300 resin.
Remarque sur l'analyse
The antibody was characterized on ceramide covalently bound to myoglobin.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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