Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

SRE0026

Sigma-Aldrich

Phosphatase substrate

Suitable for manufacturing of diagnostic kits and reagents

Synonym(s):

Phosphatase substrate, 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
333338-18-4
Molecular Weight:
371.14
EC Number:
224-246-5
MDL number:
MFCD00066288
UNSPSC Code:
12352204
PubChem Substance ID:
329826114
NACRES:
NA.32

Quality Level

400

Assay

≥97% (HPLC)

form

powder

solubility

water: 100 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Application

Phosphatase substrate has been used in the chromogenic detection of adsorbed rabbit IgG to determine coating efficiency.
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Cheng-Chia Tang et al.
eLife, 10 (2021-06-24)
Bone formation and resorption are typically coupled, such that the efficacy of anabolic osteoporosis treatments may be limited by bone destruction. The multi-kinase inhibitor YKL-05-099 potently inhibits salt inducible kinases (SIKs) and may represent a promising new class of bone
Single step, direct fluorescence immunoassays based on metal enhanced fluorescence (MEF-FIA) applicable as micro plate-, array-, multiplexing-or point of care-format
Hawa G, et al.
Analytical biochemistry, 549, 39-44 (2018)
Christina Papista et al.
Kidney international, 88(2), 276-285 (2015-03-26)
IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the
Rasmus Iversen et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(48), 17146-17151 (2014-11-19)
The multifunctional enzyme transglutaminase 2 (TG2) is the target of autoantibodies in the gluten-sensitive enteropathy celiac disease. In addition, the enzyme is responsible for deamidation of gluten peptides, which are subsequently targeted by T cells. To understand the regulation of
Masaya Yokota et al.
Arthritis research & therapy, 16(2), R72-R72 (2014-03-19)
In addition to the pivotal roles of mast cells in allergic diseases, recent data suggest that mast cells play crucial roles in a variety of autoimmune responses. However, their roles in the pathogenesis of autoimmune skeletal muscle diseases have not
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service