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Phosphatase substrate

Name: Phosphatase substrate
Brand: SIGMA

chromogenic, powder

Synonym(s):

4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

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About This Item

Linear Formula:

O₂NC₆H₄OP(O)(ONa)₂· 6H₂O

CAS Number:

333338-18-4

Molecular Weight:

371.14

NACRES:

NA.83

PubChem Substance ID:

24898495

UNSPSC Code:

12352204

EC Number:

224-246-5

MDL number:

MFCD00066288

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Properties

Product Name

Phosphatase substrate, powder

Quality Segment

200

form

powder

technique(s)

protein detection: suitable

solubility

water: 100 mg/mL, clear to slightly hazy

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

Description

Application

Phosphatase substrate has been used as a substrate in tartrate-resistant acid phosphatase (TRAP) assay and alkaline phosphatase (ALP).

Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

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This Item	SRE0026	P5994	71768
Sigma-Aldrich P4744 Phosphatase substrate	Sigma-Aldrich SRE0026 Phosphatase substrate	Sigma-Aldrich P5994 Phosphatase substrate	Sigma-Aldrich 71768 4-Nitrophenyl phosphate disodium salt hexahydrate
description powder	description Suitable for manufacturing of diagnostic kits and reagents	description 40 mg tablets	description suitable for enzyme immunoassay, ≥99.0% (enzymatic)
solubility water: 100 mg/mL, clear to slightly hazy	solubility water: 100 mg/mL, clear to slightly hazy	solubility H₂O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)	solubility -
Quality Level 200	Quality Level 400	Quality Level 200	Quality Level 100
form powder	form powder	form tablet	form powder or crystals
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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