SAB5201916

Monoclonal Anti-ERp57-Atto 488 antibody produced in mouse

clone 4F9, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-PDIA3
• UNSPSC Code: 12352203
• NACRES: NA.45

Properties

• biological source: mouse
• conjugate: Atto 488 conjugate
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: 4F9; monoclonal
• description: clone 4F9, purified immunoglobulin, buffered aqueous solution; target molecular weight: 57 kDa
• form: buffered aqueous solution
• species reactivity: human (Tested)
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles; protect from light
• concentration: 1.0 mg/mL
• technique(s): immunofluorescence: 1:100; western blot: 1:1000
• isotype: IgG2b
• UniProt accession no.: P30101
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PDIA3(2923)

Description

Immunogen

Immunogen Description: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)

Immunogen Region: Other – provide in Additional Product Details

Immunogen Species: Human

Immunogen type: Recombinant protein

Application

Western blotting
Dilution 1:1000
Tissue/Cell Type Used: HeLa cell lysate

Immunofluorescence
Dilution 1:100
Tissue/Cell Type Used: HeLa

Physical form

50% glycerol, 0.09% Sodium Azide

PBS pH 7.4

Preparation Note

Derived from cell/tissue culture

Storage and Stability

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term.

Other Notes

Additional Product Details:
Immunogen region: Amino acids 25-505
Lot Expiration/Retest Date: Expires 1 year from purchase

Target/Gene Description: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface . ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus .
Purification method: Protein G purified
Product type: Primary antibody
Antibody structure: Whole Antibody
Protein ID: NP_005304.3

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2