SAB4200053

Anti-DNA polymerase δ p125 antibody, Rat monoclonal

clone PDG 5G1, purified from hybridoma cell culture

• Synonym(s): Anti-POLD1, Anti-Polymerase (DNA directed), delta 1, catalytic subunit 125kDa, Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: PDG 5G1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~125 kDa
• species reactivity: human, mouse, rat
• packaging: antibody small pack of 25 μL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using Jurkat cells extract
• UniProt accession no.: P28340
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... POLD1(5424)

Description

General description

Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.

Immunogen

human p125

Application

Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• co-immunoprecipitation

Biochem/physiol Actions

The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable