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Key Documents

RAB0036

Sigma-Aldrich

Human Carbonic Anhydrase IX ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

CA9

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.2 pg/mL
standard curve range: 0.205-50 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CA9(768)

Related Categories

General description

Carbonic anhydrase IX (CA9) belongs to the zinc-containing metalloenzymes family. The protein is encoded by the gene mapped to human chromosome 9p. The encoded protein is localized on cell membrane and is characterized with an N-terminal proteoglycan-like domain (PG), a CA catalytic domain (CA), a transmembrane helical segment and a short intra-cytoplasmic tail. CA9 is expressed at very low levels in normal tissues (mostly in gastrointestinal tract), but it is expressed at high level in various cancer tissues, such as renal, lung, cervical, ovarian, esophageal and breast cancer.
The Human CA-IX (Carbonic Anhydrase IX) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-IX in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CA9

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Carbonic anhydrase IX (CA9) aids in reversible hydration of carbon dioxide to carbonic acid and bicarbonate. CA9 functions as a promising biomarker for diagnosing clear cell renal cell carcinoma (ccRCC), tumor hypoxia and prostate cancer (PC). In addition to pH control, CA9 also plays a vital role in cell proliferation, adhesion and tumor invasion.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J E Lake et al.
HIV medicine, 15(7), 431-441 (2014-02-11)
Soluble CD14 (sCD14) is a monocyte activation marker associated with increased mortality in HIV infection. We assessed 48-week changes in sCD14 and other inflammatory biomarkers in virologically suppressed, HIV-infected women switching to raltegravir (RAL) from a protease inhibitor (PI) or
Biochemical characterization of CA IX, one of the most active carbonic anhydrase isozymes.
Hilvo M
The Journal of Biological Chemistry, 283(41), 27799-27809 (2008)
CA9 as a biomarker in preoperative biopsy of small solid renal masses for diagnosis of clear cell renal cell carcinoma.
Li G
Biomarkers, 22(2), 123-126 (2017)
D S Pisetsky et al.
Cytokine, 69(1), 110-115 (2014-07-16)
Anorexia nervosa (AN) is a serious, potentially life-threatening disorder characterized by severe weight loss, dysregulated eating, and often excessive exercise. While psychiatric illnesses such as depression are associated with increased levels of pro-inflammatory mediators, evidence for such disturbances in patients
Chromosome 9p deletions identify an aggressive phenotype of clear cell renal cell carcinoma.
La Rochelle J
Cancer, 116(20), 4696-4702 (2010)
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