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MAK124

Sigma-Aldrich

BCG Albumin Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Bromocresol Green Albumin Assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 250 colorimetric tests

detection method

colorimetric

relevant disease(s)

genitourinary disorders; gastrointestinal diseases

storage temp.

−20°C

Gene Information

human ... ALB(213)
mouse ... ALB(11657)
rat ... ALB(24186)

General description

Albumin is the most abundant plasma protein in humans. It accounts for ∼60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure and binding of key substances such as long-chain fatty acids, bile acids, bilirubin, hematin, calcium, and magnesium. It has antioxidant and anticoagulant effects, acts as a carrier for nutritional factors and drugs, and is an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition, and protein-losing enteropathies. High levels are associated with dehydration.

Application


  • LncRNA ZEB1-AS1 inhibits renal fibrosis in diabetic nephropathy by regulating the miR-217/MAFB axis.: This research utilizes the BCG Albumin Assay Kit to quantitatively analyze albumin levels in studies of diabetic nephropathy. The results demonstrate the regulatory role of lncRNA on miR-217/MAFB axis, contributing significantly to the understanding and potential treatments of kidney fibrosis in diabetic patients (Song et al., 2019).

  • Albumin depletion of human plasma also removes low abundance proteins including the cytokines.: This study employs the BCG Albumin Assay Kit to investigate the effects of albumin depletion in human plasma, specifically its impact on the presence of low abundance proteins such as cytokines. The findings are crucial for the refinement of proteomic analyses and understanding protein interactions in biological samples (Granger et al., 2005).


Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of Albumin in serum, plasma, urine, and other biological samples.

Principle

The BCG (Bromocresol Green) albumin assay kit is designed to measure albumin directly without any pretreatment of samples. The optimized formulation substantially reduces interference by other substances (lipids/other proteins) in the raw samples. It may also be used to measure effects of drugs and other compounds on albumin metabolism. The procedure involves addition of a single working reagent and a 5 minute incubation. The optimized formulation has greatly enhanced reagent and signal stability. The kit utilizes bromocresol green, which forms a colored complex specifically with albumin. The intensity of the color, measured at 620 nm, is directly proportional to the albumin concentration in the sample.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
124CE11A15
124CE07A03
124CE07A03
124CE11A15
124CD06A08

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Marvin Gonzalez-Quiroz et al.
BMC nephrology, 20(1), 21-21 (2019-01-18)
After two-years of follow-up of 263 apparently healthy 18- to 30-year-old men in communities affected by Mesoamerican nephropathy (MeN), we identified three distinct case groups: a subgroup with (i) established renal dysfunction (case-group 1); individuals with (ii) a rapid decline in
Alaa T Alshareeda et al.
PloS one, 12(8), e0184004-e0184004 (2017-08-30)
Hepatocellular carcinoma (HCC) is considered the 3rd leading cause of death by cancer worldwide with the majority of patients were diagnosed in the late stages. Currently, there is no effective therapy. The selection of an animal model that mimics human
Maria Callejo et al.
Journal of clinical medicine, 9(2) (2020-02-12)
Introduction: Epidemiological studies suggest a relationship between vitamin D deficiency and cardiovascular and respiratory diseases. However, whether total, bioavailable, and/or free vitamin D levels have a prognostic role in pulmonary arterial hypertension (PAH) is unknown. We aimed to determine total
Jonathan A Young et al.
Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society, 46-47, 5-15 (2019-05-13)
Growth hormone (GH) has been reported to enhance the intestinal barrier; as such, recombinant GH has been administered for several intestinal diseases. However, excess GH action has been implicated in increasing the risk of intestinal dysfunction. The goal of this
Samantha V Llewellyn et al.
Journal of nanobiotechnology, 19(1), 193-193 (2021-06-30)
With the continued integration of engineered nanomaterials (ENMs) into everyday applications, it is important to understand their potential for inducing adverse human health effects. However, standard in vitro hazard characterisation approaches suffer limitations for evaluating ENM and so it is
View All Related Papers

Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Has the kit been tested with baboon and porcine samples, or is the kit not species-specific?

    1 answer

    1. The kit has been validated for use with multiple species, such as mice, rats, humans, sheep, macaques, and baboons. To date, there are no reports of its application in testing with pig samples. Details of its use in baboon studies have been documented; however, the specific reference has been removed as per the guidelines provided.

      Helpful?

  4. Is the standard curve for MAK124 BCG Albumin Assay Kit expected to be a curved or a straight line? Also, what are the typical absorbances for the curve?

    1 answer

    1. As per the information, the curve requires a non-linear curve fit. For the data in the protocol sheet, a one-site binding hyperbola function in the program Graphpad Prism was used. It is uncertain whether this function is available in Excel. Typically, a standard curve will top out at around OD = 1, while the blank is around OD = 0.20. The 5 g/dL standard is simply 5% bovine serum albumin in solution and should not be traceable to another NIST standard.

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