The kit has been validated for use with multiple species, such as mice, rats, humans, sheep, macaques, and baboons. To date, there are no reports of its application in testing with pig samples. Details of its use in baboon studies have been documented; however, the specific reference has been removed as per the guidelines provided.
MAK124
BCG Albumin Assay Kit
sufficient for 250 colorimetric tests
Synonym(s):
Bromocresol Green Albumin Assay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
usage
sufficient for 250 colorimetric tests
detection method
colorimetric
relevant disease(s)
genitourinary disorders; gastrointestinal diseases
storage temp.
−20°C
Gene Information
human ... ALB(213)
mouse ... ALB(11657)
rat ... ALB(24186)
General description
Albumin is the most abundant plasma protein in humans. It accounts for ∼60% of the total serum protein. Albumin plays important physiological roles, including maintenance of colloid osmotic pressure and binding of key substances such as long-chain fatty acids, bile acids, bilirubin, hematin, calcium, and magnesium. It has antioxidant and anticoagulant effects, acts as a carrier for nutritional factors and drugs, and is an effective plasma pH buffer. Serum albumin is a reliable prognostic indicator for morbidity and mortality, liver disease, nephritic syndrome, malnutrition, and protein-losing enteropathies. High levels are associated with dehydration.
Application
- LncRNA ZEB1-AS1 inhibits renal fibrosis in diabetic nephropathy by regulating the miR-217/MAFB axis.: This research utilizes the BCG Albumin Assay Kit to quantitatively analyze albumin levels in studies of diabetic nephropathy. The results demonstrate the regulatory role of lncRNA on miR-217/MAFB axis, contributing significantly to the understanding and potential treatments of kidney fibrosis in diabetic patients (Song et al., 2019).
- Albumin depletion of human plasma also removes low abundance proteins including the cytokines.: This study employs the BCG Albumin Assay Kit to investigate the effects of albumin depletion in human plasma, specifically its impact on the presence of low abundance proteins such as cytokines. The findings are crucial for the refinement of proteomic analyses and understanding protein interactions in biological samples (Granger et al., 2005).
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
Suitability
Suitable for the detection of Albumin in serum, plasma, urine, and other biological samples.
Principle
The BCG (Bromocresol Green) albumin assay kit is designed to measure albumin directly without any pretreatment of samples. The optimized formulation substantially reduces interference by other substances (lipids/other proteins) in the raw samples. It may also be used to measure effects of drugs and other compounds on albumin metabolism. The procedure involves addition of a single working reagent and a 5 minute incubation. The optimized formulation has greatly enhanced reagent and signal stability. The kit utilizes bromocresol green, which forms a colored complex specifically with albumin. The intensity of the color, measured at 620 nm, is directly proportional to the albumin concentration in the sample.
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Has the kit been tested with baboon and porcine samples, or is the kit not species-specific?
1 answer-
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Is the standard curve for MAK124 BCG Albumin Assay Kit expected to be a curved or a straight line? Also, what are the typical absorbances for the curve?
1 answer-
As per the information, the curve requires a non-linear curve fit. For the data in the protocol sheet, a one-site binding hyperbola function in the program Graphpad Prism was used. It is uncertain whether this function is available in Excel. Typically, a standard curve will top out at around OD = 1, while the blank is around OD = 0.20. The 5 g/dL standard is simply 5% bovine serum albumin in solution and should not be traceable to another NIST standard.
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