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Key Documents

b.End5

96091930, mouse brain, Endothelial-like

Synonym(s):

Brain-derived Endothelial 5 Cells, bEnd3 Cells, bEnd5 Cells

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About This Item

UNSPSC Code:
41106514

product name

b.End5, 96091930

biological source

mouse brain

growth mode

Adherent

karyotype

Not specified

morphology

Endothelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse Balb/c brain endothelioma

Cell Line Description

The cell line b.End5 has been established from brain endothelial cells of BALB/c mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End5 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as CAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.

Application

Study of endothelial cell growth, differentiation and influence of oncogenes.

Culture Medium

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificates of Analysis (COA)

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