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Key Documents

B5934

Supelco

Barbital Buffer

Synonym(s):

Barbital buffer solution, Sodium barbital buffer solution

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About This Item

MDL number:
MFCD00130602
UNSPSC Code:
12161703
NACRES:
NA.32

description

When reconstituted to 1 liter each vial produces a 1× running buffer containing 60 mM sodium barbital, pH 8.6.

Quality Level

100

form

powder

drug control

Home Office Schedule 3; psychotrope (France); kontrollierte Droge in Deutschland; (Spain); Decreto Lei 15/93: Tabela IV (Portugal)

Application

Barbital buffer has been used:
  • to cast 1% agarose gels and to run agarose gel electrophoresis
  • to perform electrophoresis for lipoprotein-X (Lp-X) and lipoprotein separation
  • to perform electrophoresis for lipoprotein analysis

Biochem/physiol Actions

The most commonly used buffer for immunoelectrophoresis or immunodiffusion of protein on agarose gels or cellulose acetate. It is also used for separation of serum proteins, lipoproteins and isoenzymes.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3

Target Organs

Nervous system, Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marcelo J A Amar et al.
Pharmacology research & perspectives, 8(1), e00554-e00554 (2020-01-02)
Familial lecithin:cholesterol acyltransferase (LCAT) deficiency (FLD) is a rare genetic disease characterized by low HDL-C levels, low plasma cholesterol esterification, and the formation of Lipoprotein-X (Lp-X), an abnormal cholesterol-rich lipoprotein particle. LCAT deficiency causes corneal opacities, normochromic normocytic anemia, and
Maria C de Beer et al.
Journal of lipid research, 51(11), 3117-3125 (2010-07-30)
Serum amyloid A (SAA) is an acute-phase protein mainly associated with HDL. To study the role of SAA in mediating changes in HDL composition and metabolism during inflammation, we generated mice in which the two major acute-phase SAA isoforms, SAA1.1
Sophie Stukas et al.
Journal of lipid research, 55(8), 1721-1729 (2014-06-22)
A key step in plasma HDL maturation from discoidal to spherical particles is the esterification of cholesterol to cholesteryl ester, which is catalyzed by LCAT. HDL-like lipoproteins in cerebrospinal fluid (CSF) are also spherical, whereas nascent lipoprotein particles secreted from

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