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C3041

Sigma-Aldrich

Carbonate-Bicarbonate Buffer

capsule

Synonym(s):

Carbonate Buffer Solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

form

capsule

Quality Level

200

pH

9.3-9.9

solubility

water, high purity: 1 capsule/100 mL

application(s)

diagnostic assay manufacturing

General description

Bicarbonate buffer mostly have a pH close to 7.4. It is the major small intestine buffer.

Application

Carbonate-Bicarbonate Buffer has been used for suspending the lyophilized human plasma sample for microtiter plate biofilm assay and flow cell biofilm assay. It has also been used as a coating buffer for microtiter plates prior to enzyme-linked immunosorbent assay(ELISA).
Prepared buffer is suitable for use in protein coating procedures on multiwell plates or plastic tubes.

Reconstitution

Empty the contents of one capsule in 100 mL of deionized water and dissolve. The contents of one capsule yields 100 ml of 0.05 M carbonate-bicarbonate buffer, pH 9.6 at 25 °C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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A simple, reproducible sandwich, ELISA was developed to measure porcine alpha-1 acid glycoprotein (pAGP, ORM-1) in pig plasma. Porcine AGP isolated from serum was purchased and a polyclonal antisera was prepared in rabbits using the whole pAGP molecule as immunogen.
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Developmental and Comparative Immunology, 31(2), 188-201 (2007)
L von Gersdorff Jørgensen et al.
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ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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