A7554

Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse

clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(s): Monoclonal Anti-Bovine IgG
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: alkaline phosphatase conjugate
• Clone: BG-18, monoclonal
• Application: DB, ELISA (d), IHC (p), WB CL
• Citations: 11

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: BG-18, monoclonal
• form: buffered aqueous glycerol solution
• species reactivity: bovine (non-reactive to IgM)
• should not react with: pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat
• technique(s): direct ELISA: 1:25,000, dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG, western blot (chemiluminescent): 1:100,000
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:

• western blotting
• enzyme linked immunosorbent assay (ELISA)
• dot blot
• immunohistochemistry

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

bovine IgG1 and IgG2

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)