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A7554

Sigma-Aldrich

Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse

clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Monoclonal Anti-Bovine IgG

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

BG-18, monoclonal

form

buffered aqueous glycerol solution

species reactivity

bovine (non-reactive to IgM)

should not react with

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

technique(s)

direct ELISA: 1:25,000
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG
western blot (chemiluminescent): 1:100,000

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.

Specificity

bovine IgG1 and IgG2

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunohistochemistry

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pamela Nicholson et al.
Anaerobe, 56, 78-87 (2019-02-17)
Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the
Pamela Nicholson et al.
Anaerobe, 56, 78-87 (2019-02-17)
Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the
Haet Sal Jeon et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 27(3), 344-351 (2015-04-10)
Antibody responses are useful indicators of Mycobacterium bovis infection in cattle. Many studies have evaluated the ability of immunoglobulin G (IgG) to serodiagnose bovine tuberculosis (TB). In the current study, immunoglobulin A (IgA) and IgG responses against the MPB70 and
Nasrin Husseini et al.
Translational animal science, 6(1), txac006-txac006 (2022-03-10)
The high immune response (HIR) methodology measures the genetic performance of the adaptive immune system to identify and breed animals with balanced and robust immunity. The HIR methodology has previously been used in dairy and swine to reduce disease but
Genetic and immunologic analyses of PlpE, a lipoprotein important in complement-mediated killing of Pasteurella haemolytica serotype 1
Pandher K, et al.
Infection and Immunity, 66(12), 5613-5619 (1998)
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