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Merck

11814389001

Roche

Red Blood Cell Lysis Buffer

solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions

Synonym(s):

RBC Lysis Buffer, lysis buffer, red blood cells

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About This Item

UNSPSC Code:
23201100

Pricing and availability is not currently available.

form

solution

usage

sufficient for 50-500 reactions

packaging

pkg of 100 mL

manufacturer/tradename

Roche

storage temp.

2-8°C

General description

Used for both DNA and RNA isolation, the buffer is designed for the preferential lysis of red blood cells from human whole blood, yielding intact white blood cells (free of red blood cells) for further applications. This buffer is not intended for use with whole blood from any other species.

Application

Red blood cell lysis buffer has been used in the isolation of PBMCs (peripheral blood mononuclear cells) from HIV infected patients.[1] It has also been used in the isolation of lung cellsm,[2] naive CD8+ T cells[3] and infected cells from the lung.[4]
Used for both DNA and RNA isolation, the buffer is designed for the preferential lysis of red blood cells from human whole blood, yielding intact white blood cells (free of red blood cells) for use in further research applications.
It has been used for the lysis of blood cells in femoral bone marrow, PBMC (peripheral blood mononuclear cells) and human lung adenocarcinoma samples.[5][6][7]

Physical form

Ready-to-use solution.

Other Notes

For life science research only. Not for use in diagnostic procedures.

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available


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Quantification of membrane transporter proteins in human lung and immortalized cell lines using targeted quantitative proteomic analysis by isotope dilution nanoLC?MS/MS
Fallon JK, et al.
Journal of Pharmaceutical and Biomedical Analysis, 154, 150-157 (2018)
Dual Specificity Phosphatase 5 Is Essential for T Cell Survival
Kutty RG, et al.
PLoS ONE, 11(12), 0167246-0167246 (2016)
A virus-like particle vaccine candidate for influenza A virus based on multiple conserved antigens presented on hepatitis B tandem core particles
Ramirez A, et al.
Vaccine (2018)
Ultra-Fast Analysis of Plasma and Intracellular Levels of HIV Protease Inhibitors in Children: A Clinical Application of MALDI Mass Spectrometry
Van Kampen JJ, et al.
PLoS ONE, 5(7), 11409-11409 (2010)
Jeroen J A van Kampen et al.
PloS one, 5(7), e11409-e11409 (2010-07-14)
HIV protease inhibitors must penetrate into cells to exert their action. Differences in the intracellular pharmacokinetics of these drugs may explain why some patients fail on therapy or suffer from drug toxicity. Yet, there is no information available on the

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