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Merck

11383221001

Roche

BCIP

solution, >95% (HPLC), pkg of 3 mL (150 mg)

Synonym(s):

4-toluidine salt

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About This Item

UNSPSC Code:
12352204

Pricing and availability is not currently available.

description

4-toluidine salt

Quality Level

Assay

>95% (HPLC)

form

solution

packaging

pkg of 3 mL (150 mg)

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

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52-55 °C (lit.)

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252 °C (dec.) (lit.)

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form

solid

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solid

Gene Information

human ... EPHX2(2053)
mouse ... Ephx2(13850)

Gene Information

-

Gene Information

-

Gene Information

-

General description

BCIP serves as a substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Application

Use BCIP in alkaline phosphatase detection:
  • Southern blot
  • Northern blot
  • Western blot[1]
  • Colony and plaque hybridization
  • In situ hybridization[2][3][4]

Quality

Function test: incubation with AP

Principle

Use of BCIP as substrate for alkaline phosphatase in combination with nitro blue tetrazolium chloride (NBT) as electron acceptor: Substrates and reaction products of alkaline phosphatase catalyzed color reaction with NBT/BCIP.

Physical form

Solution (50 mg/ml) in dimethylformamide (DMF)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 Dermal - Repr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 2


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