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M7133

Sigma-Aldrich

4-Methylumbelliferyl sulfate potassium salt

sulfatase substrate

Synonym(s):

Potassium 4-methylumbelliferyl sulfate

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About This Item

Empirical Formula (Hill Notation):
C10H7KO6S
CAS Number:
15220-11-8
Molecular Weight:
294.32
Beilstein:
3803203
EC Number:
239-272-2
MDL number:
MFCD00016970
UNSPSC Code:
12352204
PubChem Substance ID:
24897141
NACRES:
NA.32

Assay

≥99% (HPLC)

form

powder

solubility

water: 5 mg/mL, clear, colorless

fluorescence

λex 334 nm; λem 370 nm (pH 10.4)
λex 360 nm; λem 449 nm (Reaction products)

storage temp.

−20°C

SMILES string

[K+].CC1=CC(=O)Oc2cc(OS([O-])(=O)=O)ccc12

InChI

1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1

InChI key

CSOCSPXOODWGLJ-UHFFFAOYSA-M

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General description

4-Methylumbelliferyl sulfate (4MUS), a general substrate, is a polar metabolite of 4-methylumbelliferone (4MU).

Application

4-Methylumbelliferyl sulfate potassium salt has been used as a substrate in coupled fluorescence sulfotransferase assay to regenerate 3′-phosphoadenosine 5′-phosphosulfate (PAPS) from 3′-phosphoadenosine 5′-phosphate for cerebroside sulfotransferase (CST) enzyme activity measurements. It has also been used as a substrate in arylsulfatase assay/4-methylumbelliferyl sulfate (4-MUS) assay to test the enzymatic activity of the protein-bead complex.

Biochem/physiol Actions

4-Methylumbelliferyl sulfate (4MUS) is capable of undergoing desulfation and involve in futile cycling with 4MU. It can be utilized to measure the arylsulfatase activity of several sulfatases.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Anthony D Verderosa et al.
Scientific reports, 11(1), 1569-1569 (2021-01-17)
Antibiotics are failing fast, and the development pipeline remains alarmingly dry. New drug research and development is being urged by world health officials, with new antibacterials against multidrug-resistant Gram-negative pathogens as the highest priority. Antivirulence drugs, which inhibit bacterial pathogenicity
Junichi Enokizono et al.
Drug metabolism and disposition: the biological fate of chemicals, 35(6), 922-928 (2007-03-14)
Breast cancer resistance protein (Bcrp/Abcg2) is a member of the ATP-binding cassette transporter family with the ability to transport a variety of sulfate conjugates. In the present study, the regional expression and activity of Bcrp and sulfotransferases (SULTs/Sults) were investigated
P M Edelbroek et al.
Journal of chromatography, 530(2), 347-358 (1990-09-14)
The anticoagulant phenprocoumon is mainly metabolized in humans to hydroxylated metabolites and their glucuronides. A method is described for the determination of phenprocoumon, 4'-hydroxyphenprocoumon, 6-hydroxyphenprocoumon, 7-hydroxyphenprocoumon, and their glucuronide and sulphate conjugates in human urine. Reversed-phase high-performance liquid chromatography is
Caroline J Dean et al.
Clinical chemistry, 52(4), 643-649 (2006-02-25)
Mucopolysaccharidosis type II (MPS II) is a lysosomal storage disorder related to a deficiency in the enzyme iduronate-2-sulfatase (IDS). Clinical trials of enzyme replacement therapy are in progress, but effective treatment will require screening assays to enable early detection and
Lihua Wang-Eckhardt et al.
Cells, 10(12) (2021-12-25)
Sulfatide synthesis in the human renal cancer cell line SMKT-R3 was strongly inhibited in the presence of low µM concentrations of AG-205, a progesterone receptor membrane component 1 (PGRMC1) antagonist. This was also the case in Chinese hamster ovary (CHO)
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