T6674

Tumor Necrosis Factor-α human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonym(s): hTNF-α, TNF-α
• CAS Number: 94948-59-1
• MDL number: MFCD00148692
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Tumor Necrosis Factor-α human, TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: powder
• potency: 0.02-0.3 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: ~17.4 kDa
• packaging: pkg of 5x10 μg; pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μg
• UniProt accession no.: P01375
• storage temp.: −20°C
• Gene Information: human ... TNF(7124)

Description

Application

TNF-α include stimulating growth of human fibroblasts and other cell lines activating polymorphonuclear neutrophils and osteoclasts and inducing interleukin-1, prostaglandin E2, and collagenase production.

Tumor Necrosis Factor-α (TNFα) human has been used:

• to analyze the effects of cytokine TNFα-stressed human neuronal and glial (HNG) cells[1]
• to investigate the molecular mechanisms of TNFα-mediated prolyl-4 hydroxylase α1 (P4Hα1) suppression[2]
• to induce death-receptor-mediated apoptosis in HeLa cells[3].

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in anti-tumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is cytotoxic for many transformed cells, but in normal diploid cells, it stimulates proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and induces production of several other cytokines.

Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated.

Physical form

Lyophilized from a 0.2 μm-filtered buffer solution containing 3mM Tris-HCl and 5% trehalose.

Analysis Note

The biological activity of TNF-α is measured by the cytolysis of murine L929 cells.