This product contains solids lyophilized from a solution with 10 mM sodium phosphate at pH 7.4 and 0.5 mM DTT.
GF314
TNF- α Protein, Human Recombinant Animal Free
Tumor necrosis factor alpha (TNF-α), also known as cachectin & TNFSF1A, is the prototypic ligand of the TNF superfamily. Recombinant animal free human TNF-α is manufactured using all non-animal reagents.
Synonym(s):
Tumor Necrosis Factor Alpha, TNFa, TNF-alpha, Cachectin, TNFSF1A
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
eCl@ss:
32160405
Assay:
>98% (SDS-PAGE and HPLC)
Recombinant:
expressed in E. coli
Pricing and availability is not currently available.
recombinant
expressed in E. coli
Quality Segment
assay
>98% (SDS-PAGE and HPLC)
impurities
<0.1 μg/mg protein endotoxin (<1EU/μg)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
-10 to -25°C
General description
Product Source: E. coli
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
Cross Reactivty
Cow, Monkey, Mouse, Rat, Sheep
Cow, Monkey, Mouse, Rat, Sheep
Physical form
Product is filtered through a 0.2 micron filter before lyophilization.
Recombinant animal free human TNF- α is manufactured using all non-animal reagents
Preparation Note
Store at -20°C for up to 6 months from date of receipt.
Analysis Note
Bioactivity assay: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥2 x 107 units/mg.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| description Tumor necrosis factor alpha (TNF-α), also known as cachectin & TNFSF1A, is the prototypic ligand of the TNF superfamily. Recombinant animal free human TNF-α is manufactured using all non-animal reagents. | description Animal-component free, recombinant, expressed in E. coli, suitable for cell culture | description Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells & certain other target cells. | description affinity isolated antibody |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| assay >98% (SDS-PAGE and HPLC) | assay ≥95% (SDS-PAGE) | assay >97% (SDS-PAGE) | assay - |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in wet ice |
| impurities <0.1 μg/mg protein endotoxin (<1EU/μg) | impurities ≤1.00 EU/μg endotoxin | impurities - | impurities - |
| storage temp. -10 to -25°C | storage temp. −20°C | storage temp. -10 to -25°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
Storage Class
11 - Combustible Solids
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What additives (e.g. DTT, trehalose, Histidine, Tris etc.) are included in solution prior to filtering / lyophilization?
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What is the recommended method for reconstituting item GF314, the Sigma-Aldrich TNF-α Protein, Human Recombinant Animal Free?
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The lyophilized Tumor Necrosis Factor-alpha should be reconstituted in sterile 18MΩ-cm H2O to a concentration of not less than 100µg/ml. This solution can then be further diluted to other aqueous solutions.
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