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P8398

Phospholipase D from cabbage

Type IV, lyophilized powder, ≥100 units/mg solid

Synonym(s):

Lecithinase D, Phosphatidylcholine phosphatidohydrolase, Phospholipase D from white cabbage

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About This Item

CAS Number:
9001-87-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-639-8
MDL number:
MFCD00131897
EC Number:
3.1.4.4 (BRENDA, IUBMB)
Specific activity:
≥100 units/mg solid
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type

Type IV

form

lyophilized powder

specific activity

≥100 units/mg solid

composition

Protein, ~25%

storage temp.

−20°C

General description

Phospholipase D (PLD) from cabbage comprises the C2 domain, PLD superfamily specific conserved regions (I–IV) with HxKx4Dx6GSxN (HKD) motif. Structurally, it is a barrel-shaped monomer bound to calcium ions.

Application

Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD is used in purification and kinetic studies.
The enzyme has been used for the preparation of Bodipy-phosphatidylcholine during the preparation of fluorescently labelled lipids.

Biochem/physiol Actions

Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Phospholipase D from cabbage is widely used in biocatalytic transphosphatidylation reactions.
The amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD. It hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Analysis Note

Protein determined using biuret.

Other Notes

One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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