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P4912

Phospholipase D from Streptomyces sp.

Type VII, lyophilized powder, ≥150 units/mg solid

Synonym(s):

Lecithinase D, Phosphatidylcholine phosphatidohydrolase

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About This Item

CAS Number:
9001-87-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-639-8
MDL number:
MFCD00131897
EC Number:
3.1.4.4 (BRENDA, IUBMB)
Specific activity:
≥150 units/mg solid
Biological source:
Streptomyces sp.
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biological source

Streptomyces sp.

Quality Segment

200

type

Type VII

form

lyophilized powder

specific activity

≥150 units/mg solid

storage temp.

−20°C

Application

Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies.
The enzyme from Sigma has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions. It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells.

Biochem/physiol Actions

Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Analysis Note

Protein by biuret

Other Notes

One unit will liberate 1.0 μmole of choline from 1,2-Dioleoyl-sn-glycero-3-phosphocholine per hour at pH 8.0 at 37 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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