The kit instructions are published in the product datasheet. This document is located in the 'DOCUMENTATION' section under 'More Documents'. Please use the links below to access the datasheet and the documentation section of the product page:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/138/239/mak137bul.pdf
https://www.sigmaaldrich.com/product/sigma/mak137#product-documentation
MAK137
Phospholipase D Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
PLD Activity Assay Kit
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
Gene Information
human ... PLD1(5337) , PLD2(5338) , PLD3(23646) , PLD4(122618) , PLD5(200150) , PLD6(201164)
mouse ... PLD1(18805) , PLD2(18806) , PLD3(18807) , PLD4(104759) , PLD5(319455) , PLD6(194908)
rat ... PLD1(25096) , PLD2(25097) , PLD3(361527) , PLD4(362792) , PLD5(289270) , PLD6(287366)
Related Categories
General description
Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition.[1] Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.[2]
Application
Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.[3]
Suitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
Principle
In this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Walid El Kayal et al.
Horticulture research, 4, 17042-17042 (2017-11-09)
Raspberry (Rubus spp.) is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness. In order to prolong its shelf life, an aqueous composition containing hexanal as the key active ingredient
Phospholipase D.
Su W and Michael A F
Handbook of Cell Signaling, 1167-1176 (2009)
Karthika Sriskantharajah et al.
Plants (Basel, Switzerland), 10(11) (2021-11-28)
'Honeycrisp' (Malus domestica Borkh.), a premium applecultivar, is highly susceptible to bitter pit and decline in quality during long-term storage. In order to enhance the quality, an aqueous composition containing hexanal was applied as a preharvest spray. The effects of
Design of isoform-selective phospholipase D inhibitors that modulate cancer cell invasiveness.
Scott S A, et al.
Nature Chemical Biology, 5(2), 108-108 (2009)
Przemysław Bernat et al.
Journal of plant physiology, 229, 158-163 (2018-08-11)
2,4-dichlorophenoxyacetic acid (2,4-D) is among the most commonly used herbicides applied for weed control during wheat cultivation. However, its application could affect wheat growth. The present study investigates the effect of the ascomycetous fungus Trichoderma harzianum on lipid peroxidation, phospholipids
-
Where are the Instructions for use, please?
1 answer-
Helpful?
-
-
Where are the instructions for the use of the Phospholipase D assay kit, please?
1 answer-
The Protocol for this kit is available in the product datasheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/138/239/mak137bul.pdf
Helpful?
-
-
Can the Phospholipase D Activity Assay Kit and the PLC Activity Kits be used in Human whole blood? Thanks,
1 answer-
The Phospholipase D Activity Assay Kit is suitable for use with biological samples, however whole blood has not been tested. As this is a colorimetric reaction, the resulting pink color would very likely be masked. Serum or plasma would be recommended.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
