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Aldolase from rabbit muscle

Name: Aldolase from rabbit muscle
Brand: SIGMA

ammonium sulfate suspension, 10-20 units/mg protein

Synonym(s):

D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase

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About This Item

CAS Number:

9024-52-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

4.1.2.13 (BRENDA, IUBMB)

MDL number:

MFCD00130453

Specific activity:

10-20 units/mg protein

Biological source:

rabbit muscle

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Properties

biological source

rabbit muscle

Quality Level

200

form

ammonium sulfate suspension

specific activity

10-20 units/mg protein

foreign activity

glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05%

storage temp.

2-8°C

Description

General description

Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer.

Application

Aldolase from rabbit muscle has been used:

in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay
as a standard in the characterization of metabolic enzymes from glaucomatous tissues[1]
in fructose 2,6-bisphosphate assay of human cell lines[2]

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 [3].

Biochem/physiol Actions

Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics. It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4).

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β ⁸ barrel fold [3].

Physical form

Crystalline suspension in 2.5 M (NH₄)₂SO₄, 0.01 M Tris, pH 7.5, 0.001 M EDTA

Analysis Note

Protein determined by biuret.

Other Notes

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

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1 of 1

This Item	A2714	G6751	L2500
Sigma-Aldrich A8811 Aldolase from rabbit muscle	Sigma-Aldrich A2714 Aldolase from rabbit muscle	Sigma-Aldrich G6751 α-Glycerophosphate Dehydrogenase from rabbit muscle	Sigma-Aldrich L2500 L-Lactic Dehydrogenase from rabbit muscle
specific activity 10-20 units/mg protein	specific activity ≥8.0 units/mg protein	specific activity 100-300 units/mg protein	specific activity 800-1,200 units/mg protein
biological source rabbit muscle	biological source rabbit muscle	biological source rabbit muscle	biological source -
form ammonium sulfate suspension	form lyophilized powder	form ammonium sulfate suspension	form ammonium sulfate suspension
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
foreign activity glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, triosephosphate isomerase ≤0.05%, lactic dehydrogenase ≤0.03%, pyruvate kinase ≤0.1%	foreign activity glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05%	foreign activity Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%	foreign activity pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
A8811-200UN	04061833400739
A8811-5KU	04061833627747
A8811-1KU	04061833400722
A8811-100UN	04061833400715

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