The volume is batch dependent. Here is an example.
L2500-5KU, batch 0000304021 CofA:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L2500-5KU0000304021.pdf
13.99 mg protein/ml X 877 units/mg Protein = about 12,269 units/ml
For a 5ku pack size: 5000 units / 12,269 units/ml which is about 0.4ml
L2500
L-Lactic Dehydrogenase from rabbit muscle
Type II, ammonium sulfate suspension, 800-1,200 units/mg protein
Synonym(s):
Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-617-8
MDL number:
Specific activity:
800-1,200 units/mg protein
Pricing and availability is not currently available.
type
Type II
Quality Level
form
ammonium sulfate suspension
specific activity
800-1,200 units/mg protein
mol wt
140 kDa
foreign activity
pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
shipped in
wet ice
storage temp.
2-8°C
General description
Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .
Application
L-Lactic Dehydrogenase from rabbit muscle has been used:
- for protein-binding measurement
- to detect the action of oxaloacetate decarboxylase
- to determine serum L- and D-lactate
Biochem/physiol Actions
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Lactic dehydrogenase is responsible for the conversion of pyruvate to lactate in the fermentative metabolism.
Physical form
Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 800-1,200 units/mg protein | specific activity 600-1,200 units/mg protein | specific activity ≥600 units/mg protein | specific activity ≥500 units/mg protein |
| form ammonium sulfate suspension | form lyophilized powder | form ammonium sulfate suspension | form ammonium sulfate suspension |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 140 kDa | mol wt 140 kDa | mol wt - | mol wt - |
| foreign activity pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01% | foreign activity pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01% | foreign activity pyruvate kinase ≤0.01% | foreign activity pyruvate kinase ≤0.03% |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
QC Methods
Duration of time that beef cattle are fed a high-grain diet affects the recovery from a bout of ruminal acidosis: Short-chain fatty acid and lactate absorption, saliva production, and blood metabolites
Schwaiger T, et al.
Journal of Animal Science, 91(12), 5743-5753 (2013)
Autofluorescence from NADH conformations associated with different metabolic pathways monitored using nanosecond-gated spectroscopy and spectral phasor analysis
Maltas J, et al.
Analytical Chemistry, 87(10), 5117-5124 (2015)
Nutritional Biochemistry, 233-233 (1999)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L2500-50KU | 04061832730653 |
| L2500-25KU | 04061838043429 |
| L2500-10KU | 04061838256034 |
| L2500-100KU | 04061833950067 |
| L2500-5KU | 04061838256041 |
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