Unfortunately, the long term stability of the prepared solution has not been determined. However, prepared solutions are expected to be quite stable. Similar Sodium Carbonate/Bicarbonate 20X solutions are assigned a 2 year shelf life. Provided that solutions are stored refrigerated and not contaminated, a comparable shelf life is expected. Solutions should be observed for any changes in color and turbidity. Please see the link below to review the prepared 20X Concentrate product options:
https://www.sigmaaldrich.com/substance/sodiumbicarbonatesodiumcarbonateconcentrate1234598765
C3041
Carbonate-Bicarbonate Buffer
Carbonate-Bicarbonate Buffer
capsule
Synonym(s):
Carbonate Buffer Solution
Sign Into View Organizational & Contract Pricing
Select a Size
50 CAPS
1.410,00 kr.
100 CAPS
2.520,00 kr.
All Photos(2)
Select a Size
Change View
50 CAPS
1.410,00 kr.
100 CAPS
2.520,00 kr.
About This Item
UNSPSC Code:
12161700
NACRES:
NA.25
Recommended Products
form
capsule
Quality Level
pH
9.3-9.9
solubility
water, high purity: 1 capsule/100 mL
application(s)
diagnostic assay manufacturing
General description
Bicarbonate buffer mostly have a pH close to 7.4. It is the major small intestine buffer.[1]
Application
Carbonate-Bicarbonate Buffer has been used for suspending the lyophilized human plasma sample for microtiter plate biofilm assay and flow cell biofilm assay.[2] It has also been used as a coating buffer for microtiter plates prior to enzyme-linked immunosorbent assay(ELISA).[3][4]
Prepared buffer is suitable for use in protein coating procedures on multiwell plates or plastic tubes.
Reconstitution
Empty the contents of one capsule in 100 mL of deionized water and dissolve. The contents of one capsule yields 100 ml of 0.05 M carbonate-bicarbonate buffer, pH 9.6 at 25 °C.
recommended
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Evolution of a physiological pH 6.8 bicarbonate buffer system: application to the dissolution testing of enteric coated products
Liu F, et al.
European Journal of Pharmaceutics and Biopharmaceutics, 78(1), 151-157 (2011)
Quantifying and comparing constitutive immunity across avian species
Millet S, et al.
Developmental and Comparative Immunology, 31(2), 188-201 (2007)
T J Caperna et al.
Domestic animal endocrinology, 60, 75-82 (2017-05-30)
A simple, reproducible sandwich, ELISA was developed to measure porcine alpha-1 acid glycoprotein (pAGP, ORM-1) in pig plasma. Porcine AGP isolated from serum was purchased and a polyclonal antisera was prepared in rabbits using the whole pAGP molecule as immunogen.
Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)
von Gersdorff J, et al.
Journal of Fish Diseases, 34(10), 749-755 (2011)
R M Jaafar et al.
Journal of fish diseases, 41(1), 117-123 (2017-07-15)
Teleosts are able to raise a protective immune response, comprising both innate and adaptive elements, against various pathogens. This is the basis for a widespread use of vaccines, administered as injection or immersion, in the aquaculture industry. It has been
Protocols
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
-
If I make a solution and there are reagents left, how long can it be stored? What should I do with the storage conditions?
1 answer-
Helpful?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
