The protein sequence is not determined for this product. Human IgG has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. These are present in serum at approximately 66%, 23%, 7% and 4%, respectively.
I4506
IgG from human serum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Synonym(s):
Human IgG
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10 MG
1.240,00 kr.
100 MG
3.530,00 kr.
50 MG
3.980,00 kr.
500 MG
13.530,00 kr.
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10 MG
1.240,00 kr.
100 MG
3.530,00 kr.
50 MG
3.980,00 kr.
500 MG
13.530,00 kr.
About This Item
Recommended Products
conjugate
unconjugated
Quality Level
grade
reagent grade
Assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
composition
Protein, ≥90%
technique(s)
ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable
storage temp.
2-8°C
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General description
Purified Human IgG is isolated from pooled normal human serum by fractionation and ion-exchange chromatography. The product is supplied as an essentially salt-free (less than 1% sodium), lyophilized powder.
Application
IgG from human serum was used as a standard at a concentration of 1.274 mg/ml in ELISA to determine antibody responses to Porphyromonas gingivalis and Prevotella intermedia.[1] It was used for immunolabeling of Vero, BHK and PtK2 cells at a concentration of 0.2 mg/ml.[2] IgG from human serum was used as a standard in SDS-PAGE of anti-Shigella LPS antibodies.[3]
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.[4]
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Necesito saber la secuencia de la proteína o el número para buscarla en uniprot
1 answer-
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What is the isoelectric point of this
1 answer-
This product is not tested on a lot-to-lot basis to determine the isoelectric point. However, historical data indicates an isoelectric point range of 6.1 - 8.5.
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Is product I4506, because it is derived from human serum, considered to potential biohazard?
1 answer-
As indicated in the corresponding Data Sheet at the link below: "BIOHAZARD: Handle as if capable of transmitting infectious agents". The source material is tested and found negative for antibody to HIV, antibody to HCV, and for HbsAG.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdfHelpful?
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What is the recommended storage method for item I4506, IgG from human serum, after reconstitution?
1 answer-
After reconstitution, keep aliquots at 2-8°C for short-term use (up to 4 weeks) and store the remaining aliquots at -20°C.
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What is the significance of absorbance at 280 nm (E280 1% = 14)?
1 answer-
The absorbance at 280 nm (E280 1% = 14) indicates the percent extinction coefficient (ϵ1%).
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what is the expected molecular weight under reducing conditions
1 answer-
I4506, Human IgG, is a tetrameric protein consisting of 2 light chains and 2 heavy. When run on a reducing gel, The heavy chain will be detectable at ~50kDa, and the light chain at ~25kda.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should I store my solution of Product I4506, IgG from human serum?
1 answer-
Solutions can be stored at -20 °C for at least 1 year.
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What is the extinction coefficient forProduct I4506, human IgG?
1 answer-
Protein concentration can be determined after reconstitution by absorbance at 280 nm (E280 (1%) = 14). A 1% solution is 10 mg/mL.
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Is Product I4506, IgG from human serum, tested for endotoxin?
1 answer-
No. Product No. I4506 is not tested for endotoxin levels.
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