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product name
B82, 85011408
biological source
mouse adipose tissue
growth mode
Adherent
karyotype
2n = 51
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse C3H/An sc areolar and adipose tissue
Cell Line Description
Derivative of NCTC clone 929 (Sigma Catalogue number. 88102702) obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%.
Application
Chromosome analysis and hybridisation studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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