U5258

Anti-Ubiquitin C-terminal Hydrolase L1 (RA-15) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-UCH-L1
• MDL number: MFCD07370533
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 27 kDa
• species reactivity: human, mouse, rat
• technique(s): microarray: suitable; western blot: 1:1,000-1:2,000 using cytosolic fraction (S1) of mouse brain or whole cell extract of human lung carcinoma A549 cell line; western blot: 1:5,000-1:10,000 using cytosolic fraction (S1) of rat brain
• UniProt accession no.: P09936
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... UCHL1(7345) ; mouse ... Uchl1(22223) ; rat ... Uchl1(29545)

Description

General description

Ubiquitin C-terminal hydrolase L1 (UCH-L1) is encoded by the gene mapped to human chromosome 4p13. It is a 223 amino acid deubiquitinating enzyme, expressed highly in neurons.

Immunogen

synthetic peptide corresponding to amino acids 202-216 located near the C-terminus of rat UCH-L1, conjugated to KLH. This sequence is identical in human, mouse, bovine, porcine, and guinea pig UCH-L1. No homology is found with other known UCH-L isoforms.

Application

Anti-Ubiquitin C-terminal Hydrolase L1 (RA-15) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Ubiquitin C-terminal hydrolase L1 (UCH-L1) hydrolyze C-terminal ubiquityl esters and amides in vitro, which is an essential reaction during cytoplasmic protein degradation. peptide-ubiquityl amides are the most favorable substrates. UCH-L1 also acts as a ubiquitin (Ub) ligase. Mutation in the gene is associated with the development of neurodegenerative diseases, such as Parkinson′s disease, spinocerebellar ataxia (SCA) and Huntington′s disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves