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39441

Millipore

Nitrate Reagent B

suitable for microbiology

Synonym(s):

Sulfanilic acid solution, 4-Aminobenzenesulfonic acid

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About This Item

CAS Number:
121-57-3
MDL number:
MFCD00007886
UNSPSC Code:
41171621
PubChem Substance ID:
329755871
NACRES:
NA.85

shelf life

limited shelf life, expiry date on the label

Quality Level

100

composition

acetic acid (30%), 1000 mL
sulfanilic acid, 8.0 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical
microbiology

suitability

Enterobacter spp.
Escherichia coli
Neisseria spp.
anaerobic bacteria
coliforms

SMILES string

Nc1ccc(cc1)S(O)(=O)=O

InChI

1S/C6H7NO3S/c7-5-1-3-6(4-2-5)11(8,9)10/h1-4H,7H2,(H,8,9,10)

InChI key

HVBSAKJJOYLTQU-UHFFFAOYSA-N

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Application

α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye.

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL6942
BCCJ0564
BCCD0792
BCBV9795
BCBM6557V

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Kwan Kim et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 100, 10-14 (2012-02-14)
We estimated the apparent size of the 'hot site' for surface-enhanced Raman scattering (SERS) located within the gaps between Pt nanoparticles and a flat Ag substrate. Initially, no Raman peaks were detected for 4-aminobenzenethiol (4-ABT) on a flat Ag substrate.
Han Ming Gan et al.
Microbiology (Reading, England), 158(Pt 8), 1933-1941 (2012-05-23)
The gene coding for the oxygenase component, sadA, of 4-aminobenzenesulfonate (4-ABS) 3,4-dioxygenase in Hydrogenophaga sp. PBC was previously identified via transposon mutagenesis. Expression of wild-type sadA in trans restored the ability of the sadA mutant to grow on 4-ABS. The
Han Ming Gan et al.
Chemosphere, 82(4), 507-513 (2010-11-26)
A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100 mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation
Kwan Kim et al.
The Journal of chemical physics, 135(12), 124705-124705 (2011-10-07)
A nanogap formed by a metal nanoparticle and a flat metal substrate is one kind of "hot site" for surface-enhanced Raman scattering (SERS). Accordingly, although no Raman signal is observable when 4-aminobenzenethiol (4-ABT), for instance, is self-assembled on a flat
Jinsong Zhang et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 63(11), 2752-2758 (2011-11-05)
4-Aminobenzenesulfonate (4-ABS), an aromatic amine and recalcitrant toxic pollutant, is widely used in the dye and pharmaceutical industry. Pannonibactersp. W1 is a specialized microbial strain which can efficiently degrade 4-ABS. This study shows the feasibility of using the specialized strain
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