05-1571

Anti-IGF-1R Antibody, clone 24-57

clone 24-57, from mouse

• Synonym(s): CD221 antigen, IGF-I receptor, Insulin-like growth factor I receptor, insulin-like growth factor 1 receptor, soluble IGF1R variant 1, soluble IGF1R variant 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 24-57, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000866.1
• UniProt accession no.: P08069
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... IGF1R(3480)

Description

General description

There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.

Specificity

This antibody recognizes IGF-IR.

Immunogen

Epitope: Unknown

KLH-conjugated linear peptide corresponding to human IGF-IR.

Application

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Soos, M., et al. (1992). Journal of Biological Chemistry. 267(18):12955-12963.)

Research Category
Metabolism

Research Sub Category
Insulin/Energy Signaling

This Anti-IGF-1R Antibody, clone 24-57 is validated for use in IH, IP for the detection of IGF-1R.

Quality

Evaluated by Immunohistochemistry in human placental villi and tonsil tissue.

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected IGF-1R in human placental villi and tonsil tissue.

Target description

155 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human placental villi and tonsil tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable