Direkt zum Inhalt
Merck

Lignocellulose-derived inhibitors improve lipid extraction from wet Rhodococcus opacus cells.

Bioresource technology (2015-07-05)
Kazuhiko Kurosawa, C Anthony Debono, Anthony J Sinskey
ZUSAMMENFASSUNG

Extracting lipids from oleaginous microbial cells in a cost effective and environmentally compatible manner remains a critical challenge in developing manufacturing paradigms for advanced liquid biofuels. In this study, a new approach using microbial growth inhibitors from lignocellulose-derived feedstocks was used to extract lipids efficiently from wet cell mass of the oleaginous bacterium Rhodococcus opacus MITXM-61. Nine common lignocellulose-derived inhibitors for treatment of cells prior to solvent extraction were used and evaluated for their efficiency of lipid extraction from the cells. When the inhibitors were individually examined, formic acid and furfural showed the highest extraction efficiency of lipids from wet cell mass. Multiple extractions of lipids with methanol from wet cell mass pretreated with combined common inhibitors or hardwood hydrolysate comprising lignocellulose-derived inhibitors resulted in lipid recovery of greater than 85% of total lipids, a 1.7-fold increase of lipid extraction as compared to those in the absence of the inhibitors.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
5-(Hydroxymethyl)furfural, ≥99%, FG
Sigma-Aldrich
Isopropylalkohol, ≥99.7%, FCC, FG
Sigma-Aldrich
2-Propanol, for molecular biology, BioReagent, ≥99.5%
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Sigma-Aldrich
2-Propanol, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
5-Hydroxymethyl-2-furaldehyd, 99%
Sigma-Aldrich
2-Propanol, anhydrous, 99.5%
Sigma-Aldrich
Essigsäure, for luminescence, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
4-Hydroxybenzaldehyd, 98%
Sigma-Aldrich
2-Propanol, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
Essigsäure, ≥99.5%, FCC, FG
Sigma-Aldrich
Lävulinsäure, 98%
Sigma-Aldrich
Essigsäure, natural, ≥99.5%, FG
Sigma-Aldrich
D-(+)-Xylose, ≥99%
Sigma-Aldrich
5α-Androstan-17β-ol-3-on, ≥97.5%
Sigma-Aldrich
Vanillin, ReagentPlus®, 99%
Sigma-Aldrich
Ameisensäure, ≥95%, FCC, FG
Sigma-Aldrich
2-Furaldehyd, ≥98%, FCC, FG
Sigma-Aldrich
Syringaldehyd, 98%
Sigma-Aldrich
Vanillin, natural, ≥97%, FCC, FG
Sigma-Aldrich
Lävulinsäure, ≥97%, FG
Sigma-Aldrich
Vanillin, ≥97%, FCC, FG
Sigma-Aldrich
D-(−)-Arabinose, ≥98% (GC)
Sigma-Aldrich
2-Furaldehyd, 99%
Sigma-Aldrich
D-(+)-Xylose, BioUltra, ≥99.0% (sum of enantiomers, HPLC)
Sigma-Aldrich
2-Furaldehyd, natural, ≥98%, FCC, FG
Sigma-Aldrich
5α-Androstan-17β-ol-3-on, purum, ≥99.0% (TLC)
Sigma-Aldrich
D-(+)-Xylose, ≥99% (GC)
Sigma-Aldrich
Lävulinsäure, natural, 99%, FG
Sigma-Aldrich
4-Hydroxybenzaldehyd, ≥97%, FG