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A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

Macromolecular bioscience (2015-07-03)

Fabio Salvatore Palumbo, Antonella Bavuso Volpe, Flavia Bongiovì, Giovanna Pitarresi, Gaetano Giammona

ZUSAMMENFASSUNG

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

Sigma-Aldrich

Dimethylsulfoxid, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

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Aceton, ACS reagent, ≥99.5%

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Dimethylsulfoxid, ACS reagent, ≥99.9%

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Deuteriumoxid, 99.9 atom % D

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Dimethylsulfoxid, for molecular biology

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Dimethylsulfoxid, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

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Dimethylsulfoxid, ReagentPlus^®, ≥99.5%

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1,2-Dichlorethan, ACS reagent, ≥99.0%

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Dimethylsulfoxid, anhydrous, ≥99.9%

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N-Hydroxysuccinimid, 98%

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L-Glutamin, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

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Deuteriumoxid, 99.9 atom % D, contains 0.05 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

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L-Glutamin

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Dimethylsulfoxid, BioUltra, for molecular biology, ≥99.5% (GC)

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Tetrabutylammoniumhydroxid -Lösung, 40 wt. % in H₂O

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SAFC

L-Glutamin

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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimid -hydrochlorid, ≥99.0% (AT)

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Tetrabutylammoniumhydroxid -Lösung, 1.0 M in methanol

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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimid -hydrochlorid, crystalline

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Diethylamin, ≥99.5%

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Dimethylsulfoxid, meets EP testing specifications, meets USP testing specifications