Direkt zum Inhalt
Merck
  • A novel Bruch's membrane-mimetic electrospun substrate scaffold for human retinal pigment epithelium cells.

A novel Bruch's membrane-mimetic electrospun substrate scaffold for human retinal pigment epithelium cells.

Biomaterials (2014-09-16)
Ping Xiang, Kun-Chao Wu, Ying Zhu, Lue Xiang, Chong Li, Deng-Long Chen, Feng Chen, Guotong Xu, Aijun Wang, Min Li, Zi-Bing Jin
ZUSAMMENFASSUNG

Various artificial membranes have been used as scaffolds for retinal pigment epithelium cells (RPE) for monolayer reconstruction, however, long-term cell viability and functionality are still largely unknown. This study aimed to construct an ultrathin porous nanofibrous film to mimic Bruch's membrane, and in particular to investigate human RPE cell responses to the resultant substrates. An ultrathin porous nanofibrous membrane was fabricated by using regenerated wild Antheraea pernyi silk fibroin (RWSF), polycaprolactone (PCL) and gelatin (Gt) and displayed a thickness of 3-5 μm, with a high porosity and an average fiber diameter of 166 ± 85 nm. Human RPE cells seeded on the RWSF/PCL/Gt membranes showed a higher cell growth rate (p < 0.05), and a typical expression pattern of RPE signature genes, with reduced expression of inflammatory mediators. With long-term cultivation on the substrates, RPE cells exhibited characteristic polygonal morphology and development of apical microvilli. Immunocytochemisty demonstrated RPE-specific expression profiles in cells after 12-weeks of co-culture on RWSF/PCL/Gt membranes. Interestingly, the cells on the RWSF/PCL/Gt membranes functionally secreted polarized PEDF and phagocytosed labeled porcine POS. Furthermore, RWSF/PCL/Gt membranes transplanted subsclerally exhibited excellent biocompatibility without any evidence of inflammation or rejection. In conclusion, we established a novel RWSF-based substrate for growth of RPE cells with excellent cytocompatibility in vitro and biocompatibility in vivo for potential use as a prosthetic Bruch's membrane for RPE transplantation.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Dimethylsulfoxid, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
Dimethylsulfoxid, ACS reagent, ≥99.9%
Sigma-Aldrich
Dimethylsulfoxid, for molecular biology
Sigma-Aldrich
Dimethylsulfoxid, suitable for HPLC, ≥99.7%
Sigma-Aldrich
Dimethylsulfoxid, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Dimethylsulfoxid, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Ameisensäure, reagent grade, ≥95%
Sigma-Aldrich
Ameisensäure, ACS reagent, ≥96%
Sigma-Aldrich
Dimethylsulfoxid, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
Ameisensäure, ACS reagent, ≥88%
Sigma-Aldrich
Dimethylsulfoxid, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Dimethylsulfoxid, PCR Reagent
Sigma-Aldrich
Ameisensäure, ≥95%, FCC, FG
Sigma-Aldrich
Dimethylsulfoxid, anhydrous, ≥99.9%
USP
Dimethylsulfoxid, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Ameisensäure -Lösung, BioUltra, 1.0 M in H2O
Sigma-Aldrich
Dimethylsulfoxid, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
8-Octanoyloxypyren-1,3,6-Trisulfonsäure Trinatriumsalz, suitable for fluorescence, ≥90% (HPCE)
Supelco
Dimethylsulfoxid, analytical standard
Supelco
Dimethylsulfoxid, for inorganic trace analysis, ≥99.99995% (metals basis)
Dimethylsulfoxid, European Pharmacopoeia (EP) Reference Standard