A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.

A novel functional role for MMSET in RNA processing based on the link between the REIIBP isoform and its interaction with the SMN complex.

PloS one (2014-06-14)

Fabio Mirabella, Alexander Murison, Lauren I Aronson, Christopher P Wardell, Andrew J Thompson, Sarah J Hanrahan, Jacqueline H L Fok, Charlotte Pawlyn, Martin F Kaiser, Brian A Walker, Faith E Davies, Gareth J Morgan

PMID24923560

ZUSAMMENFASSUNG

The chromosomal translocation t(4;14) deregulates MMSET (WHSC1/NSD2) expression and is a poor prognostic factor in multiple myeloma (MM). MMSET encodes two major protein isoforms. We have characterized the role of the shorter isoform (REIIBP) in myeloma cells and identified a clear and novel interaction of REIIBP with members of the SMN (survival of motor neuron) complex that directly affects the assembly of the spliceosomal ribonucleic particles. Using RNA-seq we show that REIIBP influences the RNA splicing pattern of the cell. This new discovery provides novel insights into the understanding of MM pathology, and potential new leads for therapeutic targeting.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

F8775

Sigma-Aldrich

Formaldehyd -Lösung, for molecular biology, 36.5-38% in H₂O

S3014

Sigma-Aldrich

Natriumchlorid, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

F1635

SAFC

Formaldehyd -Lösung, contains 10-15% methanol as stabilizer, 37 wt. % in H₂O

S5150

Sigma-Aldrich

Natriumchlorid -Lösung, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture

S8776

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Natriumchlorid -Lösung, 0.9% in water, BioXtra, suitable for cell culture

S5886

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Natriumchlorid, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S7653

Sigma-Aldrich

Natriumchlorid, BioXtra, ≥99.5% (AT)

252549

Sigma-Aldrich

Formaldehyd -Lösung, ACS reagent, 37 wt. % in H₂O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

59222C

SAFC

Natriumchlorid -Lösung, 5 M

47608

Sigma-Aldrich

Formaldehyd -Lösung, for molecular biology, BioReagent, ≥36.0% in H₂O (T)

71386

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Natriumchlorid -Lösung, BioUltra, for molecular biology, ~5 M in H₂O

71376

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Natriumchlorid, BioUltra, for molecular biology, ≥99.5% (AT)

204439

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Natriumchlorid, 99.999% trace metals basis

S6546

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Natriumchlorid -Lösung, 5 M

S1679

Sigma-Aldrich

Natriumchlorid, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

47083-U

Supelco

Formaldehyd -Lösung, stabilized with methanol, ~37 wt. % in H₂O, certified reference material

15512

Sigma-Aldrich

Formaldehyd -Lösung, meets analytical specification of USP, ≥34.5 wt. %

PHR1321

Supelco

Natriumchlorid, Pharmaceutical Secondary Standard; Certified Reference Material

A7502

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DL-Alanin, ≥99% (HPLC)

71387

Supelco

Natriumchlorid, reference material for titrimetry, certified by BAM, >99.5%

S6191

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Natriumchlorid, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture

47673

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Formaldehyd -Lösung, tested according to Ph. Eur.

450006

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Natriumchlorid, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

451940

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Natriumchlorid-³⁵Cl, 99 atom % ³⁵Cl

S0817

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Natriumchlorid -Lösung, 0.85%

378860

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W381810

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DL-Alanin, ≥99%, FCC, FG

71383

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Natriumchlorid, tested according to Ph. Eur.

606758

Sigma-Aldrich

Formaldehyd-¹²C -Lösung, 20% in H₂O, 99.9 atom % ¹²C

A0325000

Alanin, European Pharmacopoeia (EP) Reference Standard