X4875

Xanthinoxidase aus Kuhmilch

Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein

• Synonym(e): XOD, Xanthine:oxygen oxidoreductase
• CAS-Nummer: 9002-17-9
• EC-Nummer: 1.17.3.2.
• EG-Nummer: 232-657-6
• MDL-Nummer: MFCD00082145
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: bovine milk
• Typ: Grade IV
• Form: ammonium sulfate suspension
• Spezifische Aktivität: ≥0.1 units/mg protein
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Research area: Cell CycleXanthineoxidase (XO) is a homodimer. It is made up of two sub-units each made up of onemolybdenum and two iron-sulphur (2Fe-2S) centers.

Frühere EG-Nummer 1.1.3.22

Anwendung

Xanthine Oxidase from bovine milk has been used in superoxide dismutaseactivity assay.Xanthine oxidase may be suitable for producing amperometric biosensors and designing-flavonoid-type inhibitors against XO for treating inflammatory diseases, gout, and hyperuricemia. It may also be used in the synthesis of superoxide because of aldehyde oxidation.

Biochem./physiol. Wirkung

Xanthinoxidase ist ein molybdänhaltiges Enzym, das im Zytosol vorkommt und durch Flavonoide stark gehemmt werden kann. Es spielt eine wesentliche Rolle beim Metabolismus einiger Arzneimittel sowie von Purinen und Pyrimidinen. Es ist auch eine biologische Quelle für reaktive Sauerstoffspezies.

Although xanthine oxidase is found widely distributed in multiple tissues, it is highly expressed in endothelial and epithelial cells. Its expression was also increased in infected tissues where it was shown to have an anti-microbial effect due to its ability to generate reactive oxygen species. Xanthineoxidase plays an important role in catalyzing the hydroxylation ofhypoxanthine to Xanthine. Later, xanthine is converted to uric acid isexcreted by the kidneys.

Einheitendefinition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physikalische Form

Suspension in 2.3 M (NH₄)₂SO₄ containing 1 mM sodium salicylate

Hinweis zur Analyse

Protein determined by biuret

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)