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U3383

Sigma-Aldrich

Urease Buffer Reagent

Synonym(e):

Urea colorimetric determination assay reagent

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1 VIAL
104,00 €

104,00 €

Versandbereit am04. April 2025Details

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1 VIAL
104,00 €

About This Item

UNSPSC-Code:
12352204
NACRES:
NA.54

104,00 €

Versandbereit am04. April 2025Details

Beschreibung

When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.

Qualitätsniveau

200

Form

powder

Mol-Gew.

545 kDa

Lagertemp.

2-8°C

Allgemeine Beschreibung

Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia. [1]
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide.[2][3] It is secreted by several bacterial species, invertebrates, fungi, and plants.[3] Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections.[2] two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.[3]

Anwendung

Urease Buffer Reagent has been used:
  • to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method[4]
  • in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells[5]
  • to study the metabolic capacity of rat hepatocytes[6]

Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.

Piktogramme

Health hazard
GHS08

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000252832
SLCR3981
0000252832
SLCR3981
SLCL1109

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Die Dokumentenbibliothek aufrufen

Kang Cheng et al.
Hepatology (Baltimore, Md.), 50(4), 1194-1203 (2009-07-29)
Tracking stem/progenitor cells through noninvasive imaging is a helpful means of assessing the targeting of transplanted cells to specific organs. We performed in vitro and in vivo studies wherein adult human hepatocytes and human fetal liver stem/progenitor cells were labeled
Cristian Follmer
Phytochemistry, 69(1), 18-28 (2007-08-21)
The broad distribution of ureases in leguminous seeds, as well as the accumulation pattern of the protein during seed maturation, are suggestive of an important physiological role for this enzyme. Since the isolation and characterization of jack bean urease by
L A Winkelman et al.
Journal of dairy science, 96(12), 7565-7577 (2013-10-15)
This study investigated the effect of 2 different types of long-acting insulin on milk production, milk composition, and metabolism in lactating dairy cows. Multiparous cows (n=30) averaging 88 d in milk were assigned to one of 3 treatments in a
Gunnar Dahlén et al.
BMC oral health, 18(1), 89-89 (2018-05-20)
Urease is an enzyme produced by plaque bacteria hydrolysing urea from saliva and gingival exudate into ammonia in order to regulate the pH in the dental biofilm. The aim of this study was to assess the urease activity among oral
Simon Svane et al.
Scientific reports, 10(1), 8503-8503 (2020-05-24)
The nickel-dependent urease enzyme is responsible for the hydrolysis of urea to ammonia and carbon dioxide. A number of bacteria produce urease (ureolytic bacteria) and are associated with various infectious diseases and ammonia emissions from agriculture. We report the first
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Fragen

1-3 von 3 Fragen  

  1. What's the concentration of Jack bean Urease? I can only see the concentration of buffer.

    1 Antwort

    1. As provided, this product contains 100 units per vial. The mass weight per vial is not determined.

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  2. How many urease units does 1 vial contain?

    1 Antwort

    1. As provided, this product contains 100 units per vial.

      Hilfreich?

  3. What is the volume of water used for reconstitution of 1 vial?

    1 Antwort

    1. As mentioned on the sample Certificate of Analysis, 30 mL of purified water in one vial of this material yields a clear solution.

      Hilfreich?

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