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The mechanism of peroxidative N-dealkylation of alkylamines proceeds via one-electron oxidation to the iminium cation which reacts with water to give the N-hydroxymethyl derivative which decomposes to formaldehyde and the N-demethylated product. This reaction is normally inhibited by glutathione by
Tetramethylbenzidine (TMB) histochemistry for retrogradely transported horseradish peroxidase (HRP) has been successfully applied in electron microscopical preparations in the guinea pig visual cortex. In ultrathin sections, the TMB--HRP reaction product appears as an accumulation of typical electron-dense crystals. These crystals
Biochimica et biophysica acta, 1409(1), 50-57 (1998-11-07)
Site-directed mutagenesis was used to produce a Synechocystis mutant containing a histidine tag at the C terminus of the CP 47 protein of Photosystem II. This mutant cell line, designated HT-3, exhibited slightly above normal rates of oxygen evolution and
Journal of immunoassay, 14(1-2), 21-31 (1993-03-01)
An enzyme immunoassay for detection of hepatitis B surface antigen based on the use of 3 monoclonal antibodies (mAbs) was developed: an IgM as capture and 2 IgG1 for detection. The system biotin-streptavidin was compared with direct conjugation of mAbs
Photochemistry and photobiology, 62(1), 82-86 (1995-07-01)
After UV irradiation of rapidly frozen solutions of N,N,N',N'-tetramethylbenzidine (TMB) in dilute aqueous micellar cetyltrimethylammonium bromide at 80 K, the ESR signal corresponding to the TMB+ cation was detected indicating photoionization. This signal was monitored as a function of time
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
