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Merck

T1428

Sigma-Aldrich

apo-Transferrin bovine

BioReagent, suitable for cell culture, ≥98%

Synonym(e):

Human serotransferrin

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About This Item

CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.83

Biologische Quelle

bovine

Produktlinie

BioReagent

Assay

≥98%

Form

powder

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

iron, essentially free

Löslichkeit

H2O: 10 mg/mL

UniProt-Hinterlegungsnummer

Lagertemp.

2-8°C

Angaben zum Gen

bovine ... TF(280705)

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Allgemeine Beschreibung

apo-Transferrin is an iron-free form of transferrin that is present in circulation.

Anwendung

All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred.
apo-Transferrin bovine has been used as a component of:
  • basal medium for culturing bovine mammary epithelial cells
  • minimum essential medium α (MEM-α) for spermatogonial stem cells (SSCs) culture
  • induction medium for mesenchymal stem cell culture

Biochem./physiol. Wirkung

apo-Transferrin (apoTf) aids in preventing iron accumulation in tissues, especially in non-transfusion dependent β-thalassemia.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Monique P Gelderman et al.
Haematologica, 100(5), 611-622 (2015-01-27)
Intermediate beta-thalassemia has a broad spectrum of sequelae and affected subjects may require occasional blood transfusions over their lifetime to correct anemia. Iron overload in intermediate beta-thalassemia results from a paradoxical intestinal absorption, iron release from macrophages and hepatocytes, and
Keita Abe et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(1-2), 154-156 (2008-04-05)
We have investigated the mechanism of manganese ion uptake by apo-transferrin using a capillary electrophoresis method, and obtained clear evidence that oxidation state +3 and the binuclear unit of a manganese chelate are essential factors for the facile uptake by
Einar Vargas-Bello-Pérez et al.
Animal nutrition (Zhongguo xu mu shou yi xue hui), 5(2), 202-208 (2019-06-14)
The objective of this study was to determine how cytosolic triacylglycerols (TAG) are stored in mammary cells and whether this depends on the individual chemical configuration of fatty acids (FA). This objective was accomplished by addition of different FA to
Shanshan Li et al.
Journal of animal science and biotechnology, 7, 44-44 (2016-08-06)
The objective of this study was to study how changing the ratio of Lys to Thr, Lys to His, and Lys to Val affects the expression of lipogenic genes and microRNA (miRNA) in bovine mammary epithelial cells. Triplicate cultures with
Jia Wang et al.
Stem cell research & therapy, 10(1), 227-227 (2019-07-31)
High temperature has a very adverse effect on mammalian spermatogenesis and eventually leads to sub- or infertility through either apoptosis or DNA damage. However, the direct effects of heat stress on the development of spermatogonial stem cells (SSCs) are still

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