SRP6586

Histone H3 (1-136) human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(e): Histone H3/I, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/j, Histone H3/k, Histone H3/l
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol-Gew.: 15.5 kDa (1-136 aa)
• Verpackung: pkg of 100 and 250 μg
• UniProt-Hinterlegungsnummer: P68431
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... HIST1H3A(8350)

Beschreibung

Allgemeine Beschreibung

The mammalian chromatin is present in nucleosomes, made of histone octamers (H2A,H2B,H3,H4)₂. Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A).

Biochem./physiol. Wirkung

Histone proteins are basic building blocks of chromatin. Changes in the circulating H3 levels are associated with death in sepsis patients. Post-translational modifications of histone tails control chromatin organization and gene transcription. Trimethylated histone H3 lysine 4 (H3K4me3) is present at actively transcribed genes and it helps in promotion of gene expression. Histone H3K36 trimethylation (H3K36me3) is lost in various cancers and is linked with poor patient outcome. Dimethylated H3K9 (H3K9me2) is mainly present in euchromatin and is linked with repressed promoter areas. Histone methyltransferase G9a is responsible for hypoxic stress-mediated H3K9me2 in mammalian cells.

Physikalische Form

Lyophilized powder. Recommended buffer is 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol.

Sicherheitshinweise

• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable