SRP6256

VEGF-C human

recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE)

• Synonym(e): Flt4-L, VRP
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in HEK 293 cells
• Markierung: 6-His tagged (C-terminus)
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 0.15-0.7 μg/mL
• Mol-Gew.: calculated mol wt 15 kDa; observed mol wt 18-23 kDa (DTT-reduced. Protein migrates due to glycosylation. Thr 103 is the predicted N-terminus .)
• Verpackung: pkg of 10 μg
• Verunreinigungen: <1 EU/μg endotoxin (LAL test)
• UniProt-Hinterlegungsnummer: P49767
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... VEGF-C(7424)

Beschreibung

Allgemeine Beschreibung

Vascular endothelial growth factor C is also known as VEGFC, Flt4-L and VRP; it contains the C-terminal propeptide which has an unusual structure with tandemly repeated cysteine-rich motifs. Upon biosynthesis, VEGFC is secreted as a non-covalent momodimer in an anti-parallel fashion. VEGFC is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. The structure and function of this protein is similar to those of vascular endothelial growth factor D (VEGF-D). VEGFC may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Overexpression of VEGF-C causes lymphatics to enlarge possibly facilitates metastasis.

Physikalische Form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable