SRP6001

Procathepsin K human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(e): CTSK, CTSO, CTSO2
• CAS-Nummer: 177529-06-5
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: liquid
• Verpackung: pkg of 10 μg
• Konzentration: ~200 μg/mL
• Verunreinigungen: Endotoxin, tested
• NCBI-Hinterlegungsnummer: P43235
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... CATK(1513)

Beschreibung

Allgemeine Beschreibung

Procathepsin K is the inactive proenzyme of cathepsin K.[1] Cathepsin K is a lysosomal cysteine protease and part of the papain cysteine protease family.[2] It is expressed in osteoclasts.[3][4] The gene encoding this protein is localized on human chromosome 1q21.[5]

Biochem./physiol. Wirkung

Cathepsin K has a role in bone resorption and remodeling.[1][4] It has a triple helical collagen hydrolase activity.[6] Mutations in the gene encoding this protein have been linked to pycnodysostosis.[7]

Physikalische Form

Liquid (25 mM Tris pH 8.0, 500 mM NaCl)

Sonstige Hinweise

Procathepsin K can be activated by adjusting the pH 4.0 by adding an equal volume of 100 mM NaAc pH 3.9, 10 mM DTT, 5 mM EDTA followed by incubation for 40 min at RT. Activated mature Cathepsin is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, we recommend to add methyl methanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) and to freeze the sample in liquid nitrogen or on dry ice. The hydrophobic thiol-reactive compound MMTS modifies cysteine′s by attaching its relatively small, uncharged thiomethyl-blocking group to reactive sulfhydryl groups (Nishimura et al., 1975). This reversible reaction arrests the auto-proteolytic process. The activity of the enzyme can be restored to nearly unmodified levels by adding L-cysteine (3M excess over MMTS) to the enzyme solution.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable