SRP4835

PCNA human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE), ≥90% (HPLC)

• Synonym(e): Cyclin, MGC8367, PCNA, Proliferating cell nuclear antigen
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥90% (HPLC); ≥90% (SDS-PAGE)
• Form: liquid
• Mol-Gew.: ~29.8 kDa
• Verpackung: pkg of 5 μg
• Verunreinigungen: endotoxin, tested
• NCBI-Hinterlegungsnummer: P12004
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... PCNA(5111)

Beschreibung

Allgemeine Beschreibung

PCNA (proliferating cell nuclear antigen) gene is mapped to human chromosome 20p12.3 and encodes a scaffolding protein.[1][2] PCNA is a homotrimeric protein known to localize in the nucleus.[3] The PCNA ring has a central cavity that is large enough to allow the dsDNA to slide freely along it.[4]

Biochem./physiol. Wirkung

Proliferating cell nuclear antigen (PCNA) has its main function in DNA replication and repair mechanism. Itsignificantly maintains the longevity of mature neutrophil granulocytes.[2] Upregulation of the PCNA gene is observed in cervical squamous cell carcinoma.[5] PCNA supports cell survival by elevating metabolism flux. This gene expression is known to offer resistance to chemotherapy.[2] Expression of PCNA or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Recombinant PCNA was expressed with a 6x His tag in sf9 insect cells and purified by proprietary chromatographic techniques.

Physikalische Form

Sterile filtered; 5 μg/5 μl in 50 mM Tris-Acetate, pH 7.5, 1 mM EDTA, and 20% Glycerol.

Rekonstituierung

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.