SRP3317

ANGPTL-3 human

recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): ANG-5, ANGPT5
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in CHO cells
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: 62.0 kDa
• Verpackung: pkg of 50 μg
• Verunreinigungen: endotoxin, tested
• Farbe: white to off-white
• UniProt-Hinterlegungsnummer: Q9Y5C1
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... ANGPTL3(27329)

Beschreibung

Allgemeine Beschreibung

ANGPTL-3 (Angiopoietin like protein 3) is a member of the angiopoietin family of structurally related proteins, characterized by a coiled N-terminal domain and a C-terminal fibrinogen like domain.[1] It is mainly present in the hepatocytes and is also secreted into circulation.[2] The gene is mapped to human chromosome 1p31.[3]
Recombinant human ANGPTL-3 is a glycoprotein that migrates by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) analysis at an apparent molecular weight of 62kDa, and contains 452 amino acid residues including a C-terminal His tag.

Biochem./physiol. Wirkung

ANGPTL-3 (Angiopoietin like protein 3) can exert activities related to both angiogenesis and lipid metabolism.[4][5] ANGPTL-3 inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), which has the effect of increasing plasma levels of triglycerides and HDL (high-density lipoprotein)-associated cholesterol.[2] The fibrinogen like portion of the ANGPTL-3 protein can bind α-5/β-3 integrins leading to endothelial cell adhesion and migration.[5] Mutations in this gene can result in familial combined hypobetalipoproteinemia.[1]

Physikalische Form

Lyophilized from 10mM Sodium Phosphate, pH 7.5.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable