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SRP2161

Sigma-Aldrich

Androgen Receptor human

recombinant, expressed in insect cells, ≥70% (SDS-PAGE)

Synonym(e):

TFM

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.26

Biologische Quelle

human

Rekombinant

expressed in insect cells

Assay

≥70% (SDS-PAGE)

Form

frozen liquid

Mol-Gew.

~100 kDa

Verpackung

pkg of 5 μg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Konzentration

100 μg/mL

Farbe

clear
 colorless

NCBI-Hinterlegungsnummer

NM_000044

UniProt-Hinterlegungsnummer

P10275

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... AR(367)

Allgemeine Beschreibung

Androgen receptor (AR) gene is mapped to human chromosome Xq11- Xq12 and contains PGK1P1 pseudogene. AR gene is extended over ~5-10 million bp of genomic DNA.[1]

Biochem./physiol. Wirkung

AR (dihydrotestosterone receptor); testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease. The androgen receptor is an androgen-activated member of the nuclear receptor superfamily of transcription factors. Signaling by the androgen receptor plays a key role in proper development and function of male reproductive organs. The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. The androgen receptor (AR) has been shown to play a critical role in the development and progression of the prostate cancer.

Physikalische Form

Clear and colorless frozen liquid solution

Angaben zur Herstellung

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
018317190
111180100

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Die Dokumentenbibliothek aufrufen

Elliot R Cooper et al.
Drug testing and analysis, 13(5), 903-915 (2021-03-13)
Androgens remain abused performance-enhancing drugs in sports. Technologies based on mass spectrometry can detect all forms of androgens but fail if the androgen represents a novel structure. A bioassay detects androgens based on function rather than structure. To date, there
Androgen receptor locus on the human X chromosome: regional localization to Xq11-12 and description of a DNA polymorphism.
Brown CJ
American Journal of Human Genetics, 44, 264-269 (1989)
Rute B Marques et al.
International journal of cancer, 117(2), 221-229 (2005-05-19)
To study the mechanisms whereby androgen-dependent tumors relapse in patients undergoing androgen blockade, we developed a novel progression model for prostate cancer. The PC346C cell line, established from a transurethral resection of a primary tumor, expresses wild-type (wt) androgen receptor
Shalini Murthy et al.
The Prostate, 64(4), 362-372 (2005-03-09)
1,25(OH)2D3 inhibits the growth of prostate cancer cells; previous reports suggest that 1,25(OH)2D3 actions in LNCaP prostate cancer cells are androgen dependent. This is due in part to the observation that the androgen receptor (AR) antagonist, Casodex, modestly inhibits LNCaP
Eric Metzger et al.
Nature, 437(7057), 436-439 (2005-08-05)
Gene regulation in eukaryotes requires the coordinate interaction of chromatin-modulating proteins with specific transcription factors such as the androgen receptor. Gene activation and repression is specifically regulated by histone methylation status at distinct lysine residues. Here we show that lysine-specific

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  1. How does the storage temperature relate to shipping conditions?

    1 Antwort

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  2. What is the Department of Transportation shipping information for this product?

    1 Antwort

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. In what applications can Product SRP2161, Androgen receptor, human, recombinant, be used?

    1 Antwort

    1. Product SRP2161, Androgen receptor human, recombinant, can be used in gel mobility shift assays, protein to protein interaction assays, and small molecules to protein interaction assays.

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  4. How is Product SRP2161, Androgen receptor, human, recombinant, purified?

    1 Antwort

    1. Product SRP2161, Androgen receptor, human, recombinant, is a FLAG-tag recombinant protein that is purified by affinity chromatography, in combination with FPLC column chromatography.

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  5. What is the formulation of the buffer used in Product SRP2161, Androgen receptor, human, recombinant?

    1 Antwort

    1. The androgen receptor is dissolved in the following buffer: 20 mM Tris-HCl, pH 7.9, 100 mM NaCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.

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