SRP0283
Sphingosine kinase 1 Active human
recombinant, expressed in baculovirus, ≥80% (SDS-PAGE)
Synonym(e):
SK1, SPHK1, SPK 1
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About This Item
UNSPSC-Code:
12352204
NACRES:
NA.54
Empfohlene Produkte
Rekombinant
expressed in baculovirus
Assay
≥80% (SDS-PAGE)
Form
aqueous solution
Mol-Gew.
46.9 kDa
NCBI-Hinterlegungsnummer
Versandbedingung
dry ice
Lagertemp.
−70°C
Angaben zum Gen
human ... SPHK1(8877)
Allgemeine Beschreibung
Human Sphingosine kinase 1 (GenBank Accession No. AF200328), full length with N-terminal His tag, MW = 46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Anwendung
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Einheitendefinition
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min
Physikalische Form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Angaben zur Herstellung
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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